Application of next generation sequencing for the diagnosis of viruses and virus-like diseases of grapevine
Project goals
Public support
Provider
Ministry of Education, Youth and Sports
Programme
COST CZ
Call for proposals
COST CZ 5 (SMSM2015LD5)
Main participants
Národní centrum zemědělského a potravinářského výzkumu, v. v. i.
Contest type
VS - Public tender
Contract ID
MSMT-33315/2015-1
Alternative language
Project name in Czech
Použití next generation sequencing pro diagnostiku virových a virům podobných chorob révy vinné
Annotation in Czech
Cílem předkládaného projektu je výzkum použití metod označovaných jako Next Generation Sequencing (NGS) pro detekci a detailní charakterizaci virů révy vinné jak na úrovni jejich vlastních virových genomů, tak na úrovni interakcí s hostitelským organismem.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
GF - Diseases, pests, weeds and plant protection
CEP - secondary branch
EB - Genetics and molecular biology
CEP - another secondary branch
EE - Microbiology, virology
10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10606 - Microbiology
10607 - Virology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
30101 - Human genetics
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
Finished project aimed at a next generation sequencing (NGS) of viruses and viroids infecting grapevines to achieve knowledge about presence of those pathogens. Results of sequencing were published in 2016 in the PLoS One journal, thus reaching planned Result V001 („Study the spectrum of viruses infecting grapevines in the Czech Republic). Subsequent survey of distribution was published as a paper of Eichmeier et al., 2016 as well as a specialized map (Komínková and Komínek, 2017), thus reaching planned Result V002 („Research on distribution on newly detected viruses and viroids in the Czech Republic). In 2017, NGS analyses was conducted, using libraries of small RNA from grapevine plants cv. Rheinriesling, sanitated in vitro using Ribavirin. Non-sanitated plants of the same cultivar were used for comparison. This accomplished planned Result V003 („Using of NGS technology in sanitation of propagation material of grapevine“).
Solution timeline
Realization period - beginning
Oct 14, 2015
Realization period - end
Dec 31, 2017
Project status
U - Finished project
Latest support payment
Feb 24, 2017
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP18-MSM-LD-U/01:1
Data delivery date
Jun 12, 2018
Finance
Total approved costs
1,688 thou. CZK
Public financial support
1,688 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Recognised costs
1 688 CZK thou.
Public support
1 688 CZK thou.
0%
Provider
Ministry of Education, Youth and Sports
CEP
GF - Diseases, pests, weeds and plant protection
Solution period
14. 10. 2015 - 31. 12. 2017