Structural studies of transcriptional regulators of the DeoR and GntR families involved in catabolic repression in Bacillus subtilis.
Project goals
In many bacteria, the expression of the genes involved in the metabolism of secondary carbon sources is typically repressed in the presence of the most favorable nutrient. This mechanism of regulation, designated carbon catabolite repression, has been extensively studied in B. subtilis. It requires participation of numerous specific repressors and activators controlling the level of transcription of catabolic genes. Transcriptional repressors involved in catabolic repression mainly belong to one of thetwo large families of prokaryotic transcriptional regulators, GntR and DeoR, respectively. Members of these families share an N-terminal helix-turn-helix DNA-binding domain and and structurally dissimila C-terminal domains that are involved in effector binding and oligomerization. We have recently determined crystal structures of the effector binding domains of two transcriptional repressors from B. subtilis, CggR (central glycolytic genes regulator) and TreR (trehalose operon repressor), belonging t
Keywords
Transciptional repressorcatabolic repressionBacillus subtilisprotein crystallographyX-ray crystallography3-D structurecrystal structure
Public support
Provider
Ministry of Education, Youth and Sports
Programme
KONTAKT
Call for proposals
KONTAKT 6 (SMSM2008ME3)
Main participants
—
Contest type
VS - Public tender
Contract ID
2060/2011-320
Alternative language
Project name in Czech
Strukturní studie transkripčních regulátorů rodiny DeoR a GntR účastnících se katabolické represe v bakterii Bacillus subtilis.
Annotation in Czech
Cílem projektu je získat strukturní informaci o skupině transkripčních represorů účastnících se katabolické represe v bakterii Bacillus subtilis. K určení struktur využijeme metod rentgenové krystalografie.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
CE - Biochemistry
CEP - secondary branch
BO - Biophysics
CEP - another secondary branch
EE - Microbiology, virology
10606 - Microbiology
10607 - Virology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
10610 - Biophysics
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
The aim of a five-year project of bilateral collaboration was to obtain structural information on group of transcriptional regulators in bacteria Bacilus subtilis. Six three-dimensional structures of CggR, AraR and DeoR repressors were determined. Obtained results, which are crucial for understanding mechanisms of gene regulation, were published in scientific journals.
Solution timeline
Realization period - beginning
Jan 1, 2008
Realization period - end
Dec 31, 2012
Project status
U - Finished project
Latest support payment
Feb 16, 2012
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP13-MSM-ME-U/01:1
Data delivery date
Jun 28, 2013
Finance
Total approved costs
1,240 thou. CZK
Public financial support
1,240 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
1 240 CZK thou.
Public support
1 240 CZK thou.
100%
Provider
Ministry of Education, Youth and Sports
CEP
CE - Biochemistry
Solution period
01. 01. 2008 - 31. 12. 2012