Optical fibre based biosensor for detection of Lyme borreliosis spirochetes
Project goals
The main objective of this project is basic research of nanostructured materials for development of LMR biosensors capable to detect tick-borne pathogens Lyme borreliosis spirochetes and tick-borne encephalitis virus. The architecture of the proposed sensor is based on LMR at optical fibre coated by transparent electrically conductive film whose surface is subsequently functionalized by opened reactive group. Nanostructured surfaces containing reactive groups will be used for covalent immobilisation of antibodies and other molecules capable of detection of pathogens, metabolites, disease markers, etc. Tested will be antibodies recognizing various known disease markers and antigenic epitopes of arthropod-borne diseases, such as the tick-borne encephalitis virus (protein E) or Lyme disease causative agent B. burgdorferi s.l. spirochetes (OspA, OspB, FlaB proteins).
Keywords
bio-sensoroptical fibreplasma assisted depositionfunctional thin filmsLyme Borreliosis
Public support
Provider
Ministry of Education, Youth and Sports
Programme
Promoting the mobility of researchers and workers in the framework of international cooperation in R&D
Call for proposals
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Main participants
Jihočeská univerzita v Českých Budějovicích / Přírodovědecká fakulta
Contest type
M2 - International cooperation
Contract ID
MSMT-21470/2018-2
Alternative language
Project name in Czech
Biosenzory na optickém vlákně pro detekci spirochét Lymské boreliózy
Annotation in Czech
Hlavním cílem projektu je základní výzkum nanostrukturovaných materiálů pro LMR biosenzory, schopné detekovat klíšťaty přenášené patogeny spirochéty Lymské boreliózy a virus klíšťové encefalitidy. Navrhovaný LMR senzor je založen na optickém vlákně, opatřeném opticky transparentní a elektricky vodivou tenkou vrstvou, která je následně funkcionalizována reaktivní chemickou vazbou. Nanostrukturované povrchy s otevřenými vazbami umožní kovalentní imobilizaci protilátek a dalších molekul sloužících k detekci patogenů, metabolitů, markerů chorob atd. Testovány budou zejména protilátky schopné detekce markerů chorob a antigenních epitopů spojených s členovci přenášenými patogeny: klíšťové encefalitidy (protein E) nebo původců Lymské boreliózy spirochéty B. burgdorferi (OspA, OspB, FlaB).
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)
OECD FORD - secondary branch
20501 - Materials engineering
OECD FORD - another secondary branch
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AF - Documentation, librarianship, work with information
BC - Theory and management systems
BD - Information theory
IN - Informatics
JG - Metallurgy, metal materials
JP - Industrial processes and processing
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
This project was being realized in the framework of the MOBILITY Activity that aims primarily on establishing and strenghtening ties with foreign research institutions. The control of particular outputs is not implemented by the evalution committee, but the correctness of allocated finances and the adequacy of their use are checked.
Solution timeline
Realization period - beginning
Jan 1, 2019
Realization period - end
Dec 31, 2021
Project status
U - Finished project
Latest support payment
Apr 29, 2020
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP22-MSM-8J-U
Data delivery date
Jun 30, 2022
Finance
Total approved costs
200 thou. CZK
Public financial support
200 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
200 CZK thou.
Public support
200 CZK thou.
100%
Provider
Ministry of Education, Youth and Sports
OECD FORD
Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)
Solution period
01. 01. 2019 - 31. 12. 2021