Structural gymnastics of nucleic acids: from molecular principles through biological functions to therapeutic targets.

Provider

Ministry of Education, Youth and Sports

Programme

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Call for proposals

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Main participants

Biofyzikální ústav AV ČR, v. v. i.

Contest type

OP - EU Operational Programme

Contract ID

CZ.02.1.01/0.0/0.0/15_003/0000477

Project name in Czech

Strukturní gymnastika nukleových kyselin: od molekulárních principů přes biologické funkce k terapeutickým cílům.

Annotation in Czech

Cílem projektu je sestavení špičkového interdisciplinárního týmu schopného (na základě širokého metodického a tematického záběru) efektivně řešit komplexní problémy v oblasti výzkumu alternativních struktur nukleových kyselin.

R&D category

IF - RDI infrastructure

CEP classification - main branch

EB - Genetics and molecular biology

CEP - secondary branch

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CEP - another secondary branch

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OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)

10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>30101 - Human genetics

Provider evaluation

U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)

Project results evaluation

The project solution brought many original results on characterizing structural dynamics of non-canonical nucleic acid molecules, especially quadruplexes. Rearrangements due to DNA damage and guanine deficiency were determined. There were defined conditions for the formation of i-motifs and identified suitable sequence rules. Formation of DNA i-motifs in vivo was experimentally proven, justifying studies of DNA i-motifs as gene expression regulators. Hitherto unknown RNA components of telomerases in higher plants, Diaphoretickes, Hymenoptera and Butterflies were characterized. Using bioinformatics tools, genomes of many organisms were characterized in terms of potential occurrence of non-canonical structures. A fundamental contribution lies in development of new methods, e.g. molecular modeling or experimental characterization of DNA-low molecular weight ligand interactions in vivo. New methods will facilitate e.g. the development of drugs targeted at specific DNA or RNA structures.

Realization period - beginning

Dec 23, 2016

Realization period - end

Oct 31, 2022

Project status

U - Finished project

Latest support payment

Sep 6, 2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data delivery code

CEP23-MSM-EF-U

Data delivery date

Jul 24, 2023

Total approved costs

158,315 thou. CZK

Public financial support

153,331 thou. CZK

Other public sources

0 thou. CZK

Non public and foreign sources

7,946 thou. CZK