New technology for diagnostics of the selected pollutants in environment
Project goals
The aim of the project is to develop a new diagnostic technology for detection of some selected environmental contaminants and to introduce into production a diagnostic tool based on this new technology. This tool will be proposed as a robust, semi-quantitative, rapid and easy to be performed immunochemical test. The major attention will be focused on pesticide contaminants (e.g. methylcarbamate pesticides, atrazin, etc.) and industrial contaminants (e.g. polyaromatic hydrocarbons). The principle of thenew technology will be employment of antibodies as biospecific agents and of nanoparticles as suitable signal substances for contaminant detection in a simple and rapid test. This will be based on interaction between detected contaminant and nanoparticle-labeled agent during capillar migration while flowing over a porous membrane strip, whereas at its end the agent which did not react will be biospecifically captured in the testing zone.
Keywords
contaminantpesticidePAHdiagnosticsnanoparticlesantibodiesimmunochemical
Public support
Provider
Ministry of Industry and Trade
Programme
TIP
Call for proposals
TIP 1 (SMPO2009/01)
Main participants
—
Contest type
VS - Public tender
Contract ID
FR-TI1/313
Alternative language
Project name in Czech
Nové technologie pro diagnostiku vybraných polutantů v životním prostředí
Annotation in Czech
Předmětem projektu je vyvinutí nové technologie diagnostického postupu detekce několika vybraných kontaminantů životního prostředí a zavedení do výroby diagnostického nástroje využívajícího tuto novou technologii.Tento nástroj bude navržen jako robustní,semikvantitativní, rychlý a snadno proveditelný imunochemický test.Hlavní pozornost bude upřena na kontaminanty ze skupiny pesticidů (jako např. methylkarbamátové pesticidy, atrazin, apod.) a průmyslové kontaminanty (např. polyaromatické uhlovodíky).Podstatou nové technologie bude použití protilátek jako biospecifického činidla a nanočástic jako vhodné signální látky k detekci kontaminantu v jednoduchém a rychlém testu. Ten bude spočívat v interakci detekovaného kontaminantu a činidla označeného nanočásticí během kapilární migrace při podélném toku proužkem porézní membrány, přičemž na jejím konci bude nezreagované činidlo biospecificky zachyceno v testovací zóně.
Scientific branches
R&D category
VV - Exeperimental development
CEP classification - main branch
EI - Biotechnology and bionics
CEP - secondary branch
CE - Biochemistry
CEP - another secondary branch
—
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
20801 - Environmental biotechnology
20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
20803 - Environmental biotechnology related ethics
20901 - Industrial biotechnology
20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
30401 - Health-related biotechnology
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
30404 - Biomaterials (as related to medical implants, devices, sensors)
30405 - Medical biotechnology related ethics
40401 - Agricultural biotechnology and food biotechnology
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
The goals and material contents of the project under subject were fulfilled within the defined extent and terms.
Solution timeline
Realization period - beginning
Oct 1, 2009
Realization period - end
Sep 30, 2012
Project status
U - Finished project
Latest support payment
Apr 20, 2012
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP13-MPO-FR-U/02:2
Data delivery date
Jul 14, 2014
Finance
Total approved costs
6,853 thou. CZK
Public financial support
5,135 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
1,718 thou. CZK
Basic information
Recognised costs
6 853 CZK thou.
Public support
5 135 CZK thou.
74%
Provider
Ministry of Industry and Trade
CEP
EI - Biotechnology and bionics
Solution period
01. 10. 2009 - 30. 09. 2012