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Bioinspired Nanocomposite Structures for bone Tissue Regeneration

Project goals

Bioinspired composite materials promoting the regeneration of defective bone tissue will be prepared. Their proposed composition will imitate the real bone structure, and will combine the advantages of nanofibres, macroporous gels and bioactive inorganiccomponent. This materials will be composed of (1) organic matrix represented by relatively quickly degradable synthetic macroporous hydrogels based on methacrylates, macroporous hydrogel prepared from the  natural collagen (both further supplemented with hyaluronic acid), (2) organic fibrous component represented by collagen or polylactide nanofibre scaffolds, and (3) inorganic component ? natural or synthetic nanocrystalline hydroxyapatite or tricalcium phosphate.  All composite types will be mechanically tested. The effects of the composite materials as well as their individual components on the adhesion, growth, maturation, viability and potential immune activation of osteogenic cells (in vitro tests) will be studied.

Keywords

nanofibrescollagenporoushydrogelspolylactidehydroxyapatitetricalciumphsphate

Public support

  • Provider

    Czech Science Foundation

  • Programme

    Standard projects

  • Call for proposals

    Standardní projekty 12 (SGA02009GA-ST)

  • Main participants

  • Contest type

    VS - Public tender

  • Contract ID

    106-09-1000

Alternative language

  • Project name in Czech

    Bioinspirativní nanokompozitní struktury pro obnovu kostní tkáně

  • Annotation in Czech

    Cílem tohoto grantového projektu je příprava biologicky insipirovaného nanokompozitního materiálu pro obnovu kostní tkáně, který bude imitovat reálnou strukturu kosti a zároveň kombinovat výhody nanovláken, makroporézních gelů a bioaktivního anorganického plniva. Tento materiál bude složen z  matrice, představované jednak relativně rychle degradovatelnými hydrogely na bázi methakrylátu nebo makroporézním hydrogelem na bázi přírodního kolagenu (oba typy matrice budou obohaceny kyselinou hyaluronovou). Materiál bude dále obsahovat kolagenní nebo polylaktidová nanovlákna a anorganické plnivo (přírodní nebo syntetický hydroxyapatit příp. trikalciumfosfát). Kompozity budou mechanicky testovány a bude studován vliv celých kompozitů i jednotlivých jejich složek na adhezi, růst, proliferaci, maturaci, životaschopnost a potenciální imunitní aktivaci osteogenních buněk (in vitro testy).

Scientific branches

  • R&D category

    ZV - Basic research

  • CEP classification - main branch

    FP - Other medical fields

  • CEP - secondary branch

    EI - Biotechnology and bionics

  • CEP - another secondary branch

    JI - Composite materials

  • 20505 - Composites (including laminates, reinforced plastics, cermets, combined natural and synthetic fibre fabrics; filled composites)
    20801 - Environmental biotechnology
    20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
    20803 - Environmental biotechnology related ethics
    20901 - Industrial biotechnology
    20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation
    20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
    30106 - Anatomy and morphology (plant science to be 1.6)
    30107 - Medicinal chemistry
    30108 - Toxicology
    30109 - Pathology
    30221 - Critical care medicine and Emergency medicine
    30223 - Anaesthesiology
    30224 - Radiology, nuclear medicine and medical imaging
    30225 - Allergy
    30226 - Rheumatology
    30227 - Geriatrics and gerontology
    30229 - Integrative and complementary medicine (alternative practice systems)
    30230 - Other clinical medicine subjects
    30307 - Nursing
    30308 - Nutrition, Dietetics
    30309 - Tropical medicine
    30310 - Parasitology
    30311 - Medical ethics
    30312 - Substance abuse
    30401 - Health-related biotechnology
    30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
    30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
    30404 - Biomaterials (as related to medical implants, devices, sensors)
    30405 - Medical biotechnology related ethics
    30501 - Forensic science
    30502 - Other medical science
    40401 - Agricultural biotechnology and food biotechnology
    40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
    40403 - Agricultural biotechnology related ethics

Completed project evaluation

  • Provider evaluation

    U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)

  • Project results evaluation

    The objectives of the project were fulfilled, with an exception of cell testing in a dynamic cultivation system. The created high-ranking publications are dedicated to many projects, so that the quality and quantity of outputs is lower in relation to thecosts. Some additional prepared publications promise to improve this situation.

Solution timeline

  • Realization period - beginning

    Jan 1, 2009

  • Realization period - end

    Dec 31, 2012

  • Project status

    U - Finished project

  • Latest support payment

    Apr 1, 2012

Data delivery to CEP

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

  • Data delivery code

    CEP13-GA0-GA-U/02:3

  • Data delivery date

    May 17, 2016

Finance

  • Total approved costs

    6,216 thou. CZK

  • Public financial support

    6,216 thou. CZK

  • Other public sources

    0 thou. CZK

  • Non public and foreign sources

    0 thou. CZK