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Effect of acyclic nucleosid phosphonates and other antimetabolites against plant viruses

Project goals

The project will study the effect of synthetic inhibitors of polymerases on replication and movement of viruses in plants. The project will be the first assessment of phytotoxicity and antiviral activity of acyclic nucleosid phosphonates (ANPs), PMEA, PMEDAP, PMPA and HPMPC against plant DNA virus (CaMV) and RNA viruses (TYMV, RaMV, TuMV) with different replication mechanisms. The effect of ANPs will be followed on symptom expression, formation of virions and accumulation of viral genomes. The effect ofANPs will be compared with Ribavirin, so far the most efficient and practically used antiviral compound against plant viruses, which will be used as a standard. The project will fill the gap in our knowledge about possible elimination of DNA viruses from plants. The results could be exploited in the production of virus-free vegetatively propagated crops (potato, fruits, grapevine, hops, ornamentals), and virus-free germplasm collections used for crop breeding and protection of biodiversity.

Keywords

rostlinavirusacyklické nukleosid fosfonátyantivirotika

Public support

  • Provider

    Czech Science Foundation

  • Programme

    Standard projects

  • Call for proposals

    Standardní projekty 12 (SGA02009GA-ST)

  • Main participants

  • Contest type

    VS - Public tender

  • Contract ID

    522/09/0707

Alternative language

  • Project name in Czech

    Účinnost acyklických nukleosid fosfonátů a dalších antimetabolitů proti rostlinným virům

  • Annotation in Czech

    Vyhodnotit protivirový účinek a fytotoxicitu acyklických nukleosid fosfonátů (PMEA,PMDAP,PMPA a HPMPC) proti rostlinnému dsDNA viru a RNA virům (TYMV,RaMV a TuMV), s rozdílným replikačním mechanismem, v porovnání s Ribavirinem, pomocí nově navrženého invitro modelu na brukvovitých rostlinách.

Scientific branches

  • R&D category

    ZV - Basic research

  • CEP classification - main branch

    EE - Microbiology, virology

  • CEP - secondary branch

    GF - Diseases, pests, weeds and plant protection

  • CEP - another secondary branch

    EI - Biotechnology and bionics

  • 10606 - Microbiology
    10607 - Virology
    20801 - Environmental biotechnology
    20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
    20803 - Environmental biotechnology related ethics
    20901 - Industrial biotechnology
    20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation
    20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
    30401 - Health-related biotechnology
    30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
    30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
    30404 - Biomaterials (as related to medical implants, devices, sensors)
    30405 - Medical biotechnology related ethics
    40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
    40401 - Agricultural biotechnology and food biotechnology
    40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
    40403 - Agricultural biotechnology related ethics

Completed project evaluation

  • Provider evaluation

    U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)

  • Project results evaluation

    The project gone according to plan both in terms of expertise and in terms of disbursement of funds.

Solution timeline

  • Realization period - beginning

    Jan 1, 2009

  • Realization period - end

    Dec 31, 2011

  • Project status

    U - Finished project

  • Latest support payment

    Apr 16, 2011

Data delivery to CEP

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

  • Data delivery code

    CEP12-GA0-GA-U/02:2

  • Data delivery date

    May 2, 2016

Finance

  • Total approved costs

    2,773 thou. CZK

  • Public financial support

    2,773 thou. CZK

  • Other public sources

    0 thou. CZK

  • Non public and foreign sources

    0 thou. CZK