Activity of acidophilic sulfur-oxidizing bacteria under aerobic and anaerobic conditions
Project goals
The project proposal investigates activity of sulfur-oxidizing bacteria to obtain new results on process parameters which results in environmental acidification and toxic metal mobilization in sulfide wastes and abandoned mines. An Acidithiobacillus ferrooxidans mine isolate will be a model bacterium to study elemental sulfur oxidation. To learn more about the cell energetic profile, a method for ATP determination will be applied in relation to oxidation kinetics and assessment of an actual metabolic potential of bacteria to form sulfuric acid. The aerobic bacteria will be adapted to anaerobic sulfur oxidation to study process parameters under different physiological state of bacteria. Proteome analysis of aerobic and anaerobic bacteria will be performed to obtain knowledge on characteristic proteins which are formed during elemental sulfur oxidation and a cell switch to iron oxidation. This will provide a complex view to the metabolic control. The project can contribute to the study of physiology ofsulfur-oxidizing bacteria in relation to their role in the environment.
Keywords
bakterie oxidující sirné látkykyselé důlní vodysulfidové odpady
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
Standardní projekty 11 (SGA02008GA-ST)
Main participants
—
Contest type
VS - Public tender
Contract ID
525/08/0697
Alternative language
Project name in Czech
Aktivita acidofilních bakterií oxidujících síru za aerobních a anaerobních podmínek
Annotation in Czech
1. Adenosintrifosfát v bakterii Acidithiobacillus ferrooxidans oxidující síru. 2. Anaerobní kultury A. ferrooxidans oxidující síru. 3. Analýza proteomu aerobních a anaerobních bakterií A. ferrooxidans oxidujících síru a železo(II).
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
DM - Solid waste and its control, recycling
CEP - secondary branch
CE - Biochemistry
CEP - another secondary branch
EI - Biotechnology and bionics
10511 - Environmental sciences (social aspects to be 5.7)
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
20801 - Environmental biotechnology
20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
20803 - Environmental biotechnology related ethics
20901 - Industrial biotechnology
20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
30401 - Health-related biotechnology
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
30404 - Biomaterials (as related to medical implants, devices, sensors)
30405 - Medical biotechnology related ethics
40401 - Agricultural biotechnology and food biotechnology
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
The project gone according to plan both in terms of expertise and in terms of disbursement of funds.
Solution timeline
Realization period - beginning
Jan 1, 2008
Realization period - end
Dec 31, 2011
Project status
U - Finished project
Latest support payment
Apr 16, 2011
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP12-GA0-GA-U/02:2
Data delivery date
May 2, 2016
Finance
Total approved costs
3,277 thou. CZK
Public financial support
3,277 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
3 277 CZK thou.
Public support
3 277 CZK thou.
100%
Provider
Czech Science Foundation
CEP
DM - Solid waste and its control, recycling
Solution period
01. 01. 2008 - 31. 12. 2011