Mechanisms of R-loop-mediated DNA Replication Stress: From Basic Principles to Cancer Therapy
Public support
Provider
Czech Science Foundation
Programme
—
Call for proposals
SGA0202600003
Main participants
Ústav experimentální medicíny AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
26-22773M
Alternative language
Project name in Czech
Mechanisms of R-loop-mediated DNA Replication Stress: From Basic Principles to Cancer Therapy
Annotation in Czech
Unscheduled transcription-replication conflicts (TRCs) associated with R-loop formation are major sources of DNA replication stress and cancer development. Recent research indicates that increased oxidative stress amplifies R-loop formation and results in DNA replication stalling through PRDX2-driven replisome alterations and a replication slowdown. This significantly reshapes our understanding of R-loop formation and replication stress in cancer cells, paving the way for new studies on genome instability during tumor development. We aim to investigate the conditions that promote R-loop formation and persistent replication stalling and to explore stress tolerance pathways activated by replication stress and R-loop formation. Finally, the vulnerabilities of hypoxic cancer cells to TRC processing inhibition will be tested, both in 2D and 3D models. This project is expected to deepen our understanding of the oxidative stress and DNA replication stress interplay, potentially introducing new strategies for cancer treatment.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10608 - Biochemistry and molecular biology
OECD FORD - secondary branch
—
OECD FORD - another secondary branch
—
CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
CE - Biochemistry<br>EB - Genetics and molecular biology
Solution timeline
Realization period - beginning
Jan 1, 2026
Realization period - end
Dec 31, 2030
Project status
Z - Beginning multi-year project
Latest support payment
—
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP26-GA0-GM-R
Data delivery date
Apr 27, 2026
Finance
Total approved costs
24,998 thou. CZK
Public financial support
24,998 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK