Two-dimensional separation techniques for microscale bioanalysis
Project goals
The intention is to perform on-line coupling of two separation methods in the capillary format. Resulting two-dimensional (2D) separation is expected to have high efficiency and good detection sensitivity. High peak capacity will be achieved by a combination of electroseparation methods based on different separation principles with high degree of orthogonality. Detection sensitivity will be addressed by a laser induced fluorescence (LIF) detection in conjunction with on-line preconcentration of the sample in the first dimension of the separation. The research will be preferably focused on temperature-gradient electrofocusing method, which is rather new method combining focusing and separation effects. Application field for 2D microscale separations isin the analysis of biosamples, such as proteomic or metabolomic samples, the inherent complexity of which is a serious challenge for established one-dimensional methods such as HPLC or capillary electrophoresis.
Keywords
kapilární dvoudimenzionální separacekapilární elektroforézaprekoncentrace vzorkulaserem indukova
Public support
Provider
Czech Science Foundation
Programme
Post-graduate (doctorate) grants
Call for proposals
Postdoktorandské granty 9 (SGA02009GA1PD)
Main participants
—
Contest type
VS - Public tender
Contract ID
203/09/P485
Alternative language
Project name in Czech
Dvourozměrné separační techniky pro bioanalýzu v mikroměřítku
Annotation in Czech
Záměrem je provádění dvourozměrných separací komplexních směsí biomolekul v kapilárním měřítku založených na propojení dvou elektroseparačních metod o rozdílné selektivitě. Pro zvýšení citlivosti bude fluorescenční detekce kombinována s novými gradientovými elektrofokusačními metodami.
Scientific branches
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
The project was excellent, both in terms of expertise and in terms of disbursement of funds.
Solution timeline
Realization period - beginning
Jan 1, 2009
Realization period - end
Dec 31, 2011
Project status
U - Finished project
Latest support payment
Apr 16, 2011
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP12-GA0-GP-U/03:3
Data delivery date
May 2, 2016
Finance
Total approved costs
690 thou. CZK
Public financial support
690 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
690 CZK thou.
Public support
690 CZK thou.
100%
Provider
Czech Science Foundation
CEP
CB - Analytical chemistry, separation
Solution period
01. 01. 2009 - 31. 12. 2011