Chracterisation of NADPH oxidase from tobacco pollen and its role in regulation of polar cell expansion
Project goals
NADPH oxidases (NOX) activities play important roles in plant response to stress and were described as important for plant development, notably for the tip growth of Arabidopsis root hairs. We have recently described the importance of NOX for the growthof tobacco pollen tubes. However, the detailed mechanisms of NOX regulation and the role of NOX-derived reactive oxygen species (ROS) in cellular morphogenesis remain largerly unknown. In this project I intend to characterise further NOX signalling in tobacco pollen and study the regulation of this protein. By combining biochemical, microscopical and molecular biological approaches, this project will describe regulation of pollen NOX by phospholipid second messengers, calcium ions and small GTPases fromRop family. I will study intracellular localisation and dynamics of pollen NOX and search for novel interactors of the NOX protein. Pollen tubes and whole tobacco plants with altered expression of pollen NOX genes will also be studied. Obtained findingswill help to understand the knowledge of mechanism of pollination, that is crucial for hybrid plant production in agronomy.
Keywords
NADPH oxidasareaktivní formy kyslíkuRop GTPasyfosfolipidybuněčná polaritapylová láčka
Public support
Provider
Czech Science Foundation
Programme
Post-graduate (doctorate) grants
Call for proposals
Postdoktorandské granty 9 (SGA02009GA1PD)
Main participants
—
Contest type
VS - Public tender
Contract ID
522/09/P299
Alternative language
Project name in Czech
Charakterizace NADPH oxidasy v pylu tabáku a její úloha v regulaci polárního růstu
Annotation in Czech
Během projektu budu studovat roli NADPH oxidasy a reaktivních forem kyslíku v procesu morfogeneze rostlinných buněk. NADPH oxidasa z pylu tabáku bude molekulárně charakterizována, bude studována její buněčná lokalizace a regulace vápníkem, signálními fosfolipidy a malými GTPasami.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EB - Genetics and molecular biology
CEP - secondary branch
EA - Morphology and cytology
CEP - another secondary branch
—
10601 - Cell biology
10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
30101 - Human genetics
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
The project was excellent, both in terms of expertise and in terms of disbursement of funds.
Solution timeline
Realization period - beginning
Jan 1, 2009
Realization period - end
Dec 31, 2011
Project status
U - Finished project
Latest support payment
Apr 16, 2011
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP12-GA0-GP-U/03:3
Data delivery date
May 2, 2016
Finance
Total approved costs
983 thou. CZK
Public financial support
983 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
983 CZK thou.
Public support
983 CZK thou.
100%
Provider
Czech Science Foundation
CEP
EB - Genetics and molecular biology
Solution period
01. 01. 2009 - 31. 12. 2011