Physiological role of aphid adipokinetic hormone: its interaction with juvenile hormone and influence on vitellogenin synthesis and reproduction
Project goals
Study of physiology of insect neurohormones and their effects on the selected taxons of insect pest corresponds with basic principles of the Integrated Pest Management. The aphids (Sternorrhyncha, Aphidoidea) are suitable insect target group for this research because of their evident economic importance and with respect to their hormonally controlled reproductive polyphenism. The aim of this project is to approximate the effect of adipokinetic hormone (AKH) on vitellogenin (Vg) distribution and reproduction of the model species - the pea aphid, Acyrthosiphon pisum Harris. The interactions of the AKH with juvenile hormone (JH) will be studied subsequently. Concentration and distribution of the AKH and Vg in specific morphs of A. pisum will be monitoredwith the aid of electrophoretic, chromatographic (HPLC) and immunodetection techniques (ELISA and immunohistochemistry). These characteristics will be evaluated under normal physiological conditions and after external application of AKH or JH (JH analogue alternatively). Obtained data will be used for description of relationships between AKH and JH and their effect on vitellogenesis of A. pisum. The last point of the work will focus on the effect of AKH on reproduction of this aphid species that will beexpressed by changes in demographic characteristics.
Keywords
mšiceAcyrthosiphon pisumneurohormonyadipokinetický hormonjuvenilní hormonvitelogenin
Public support
Provider
Czech Science Foundation
Programme
Post-graduate (doctorate) grants
Call for proposals
Postdoktorandské granty 9 (SGA02009GA1PD)
Main participants
—
Contest type
VS - Public tender
Contract ID
522/09/P382
Alternative language
Project name in Czech
Fyziologický význam adipokinetického hormonu u mšic: jeho interakce s juvenilním hormonem a vliv na syntézu vitelogeninu a reprodukci
Annotation in Czech
Cílem projektu je studium fyziologie adipokinetického hormonu mšic a jeho vlivu na syntézu vitelogeninu a reprodukci a dále objasnění jeho interakcí s juvenilním hormonem. Výsledkem bude přiblížení možnosti jeho využití jako bioracionálního pesticidu proti mšicím.
Scientific branches
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
The project gone according to plan both in terms of expertise and in terms of disbursement of funds.
Solution timeline
Realization period - beginning
Jan 1, 2009
Realization period - end
Dec 31, 2011
Project status
U - Finished project
Latest support payment
Apr 16, 2011
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP12-GA0-GP-U/03:3
Data delivery date
May 2, 2016
Finance
Total approved costs
2,038 thou. CZK
Public financial support
2,038 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
2 038 CZK thou.
Public support
2 038 CZK thou.
100%
Provider
Czech Science Foundation
CEP
GF - Diseases, pests, weeds and plant protection
Solution period
01. 01. 2009 - 31. 12. 2011