Preparation and study of transgenic plants with the aim to induce and enhance rhizoremediation of polychlorinated biphenyls
Public support
Provider
Czech Science Foundation
Programme
Post-graduate (doctorate) grants
Call for proposals
Postdoktorandské granty 12 (SGA02012GA1PD)
Main participants
—
Contest type
VS - Public tender
Contract ID
P501-12-P521
Alternative language
Project name in Czech
Příprava a studium transgenních rostlin s cílem indukovat a zvýšit rhizoremediaci polychlorovaných bifenylů
Annotation in Czech
Vývoj invenčních remediačních metod pro dekontaminaci míst kontaminovaných organickými polutantyje velmi důležité. Jednou z používaných metod je biologická metoda spojující tzv. fytoremediaci a rhizoremediaci. Fytoremediace využívá zelené rostliny pro přenos, akumulaci a odstraňování polutantů z životního prostředí, při které dochází ke spolupráci s rhizosferními mikroorganismy - rhizoremediací. Cílem tohoto grantu bude příprava transgenních rostlin se zvýšenými schopnostmi podpořit rhizoremediaci polychlorovaných bifenylů. Úkolem bude příprava geneticky modifikovaných rostlin s rostlinným genem NtF3H kódujícím flavonoid-3-hydroxylasu (F3H), která katalyzuje přeměnu kaempferolu na quercetin - rostlinný sekundární metabolit. Připravené rostliny nadměrněexprimující F3H a produkující tak více quercetinu by indukovaly degradační dráhu bifenylu v rhizosferních bakteriích.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EI - Biotechnology and bionics
CEP - secondary branch
EB - Genetics and molecular biology
CEP - another secondary branch
—
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>20801 - Environmental biotechnology<br>20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management<br>20803 - Environmental biotechnology related ethics<br>20901 - Industrial biotechnology<br>20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation<br>20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials<br>30101 - Human genetics<br>30401 - Health-related biotechnology<br>30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)<br>30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])<br>30404 - Biomaterials (as related to medical implants, devices, sensors)<br>30405 - Medical biotechnology related ethics<br>40401 - Agricultural biotechnology and food biotechnology<br>40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming<br>40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
O - Nesplněno zadání, smlouva však byla dodržena
Project results evaluation
According to results described, plants transformed with gene for flavonoid-3-hydroxylase were obtained. Such plants induce degration pathway of bifenyl involving rhizobacteria.These results were, however, not published. One methodological publication with impact factor on a different issue than the subject of this research was released, and eight conference papers.
Solution timeline
Realization period - beginning
Jan 1, 2012
Realization period - end
Dec 31, 2014
Project status
U - Finished project
Latest support payment
Mar 31, 2014
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP15-GA0-GP-U/02:2
Data delivery date
May 6, 2016
Finance
Total approved costs
1,231 thou. CZK
Public financial support
1,231 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK