Role of the sex chromosome W in sex determination of Lepidoptera
Project goals
Lepidoptera possess a WZ/ZZ (felame/male) sex chromosome system or its numerical variations. However, little is known about the sex-determining mechanism in this large insect order. Our recent results, obtained by comparative genomic hybridization (CGH)in Bombyx mori, Ephestia kuehniella, and Galleria mellonella suggest an accumulation of common repetitive sequences in the W chromosome. In addition, we found a male killing activity in a W-chromosome segment of Ephestia mutants. We propose here a detailed analysis of the W chromosome in selected species by methods of molecular cytogenetics. We intend to study molecular differentiation of the W by CGH, molecular composition of the W by fluorescence in situ hybridization (FISH) mapping of probes derivedeither from Bombyx retrotransposones or from W-chromatin, and pattern of histone acetylation in W and Z.It is expected that results obtained will contribute to understanding the role of W chromosome in sex determination of Lepidoptera.
Keywords
Lepidopterasex chromosomessex determinationheterochromatinW chromatinmolecular cytogeneticscomparative genomic hybridizationCGHfluorescence in situ hybridizationFISHrepetitive DNA sequencesretrotransposoneshistone acetylation
Public support
Provider
Academy of Sciences of the Czech Republic
Programme
Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the Academy of Sciences of the Czech Republic
Call for proposals
Výzkumné granty 3 (SAV02003-A)
Main participants
Entomologický ústav AV ČR
Contest type
VS - Public tender
Contract ID
—
Alternative language
Project name in Czech
Role pohlavního chromosomu W v determinaci pohlaví motýlů
Annotation in Czech
Lepidoptera mají WZ/ZZ (samice/samec) systém pohlavních chromosomĎ nebo jeho numerické variace. Avçak velmi málo je známo o mechanismu determinace pohlaví u tohoto velkého ýádu hmyzu. Naçe nedávné věsledky, získané komparativní genomovou hybridizací (CGH) u Bombyx mori, Ephestia kuehniella a Galleria mellonella, indikují akumulací bŘÔněch repetitivních sekvencí v chromosomu W. TéÔ jsme v segmentu W chromosomu u mutantu Ephestia prokázali pýítomnost faktoru letálního pro samčí pohlaví. Navrhujeme detailní analězu chromosomu W u vybraněch druhů metodami molekulární cytogenetiky. Hodláme studovat molekulární diferenciaci W pomocí CGH, molekulární složení W mapování sond odvozeněch z retrotransposonů B. mori či z W-chromatinu fluorescenční in situ hybridizací (FISH), a také acetylaci histonů u W a Z. Očekáváme, že získané výsledky přispějí k poznání role chromosomu W v determinaci pohlaví motýlů.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EB - Genetics and molecular biology
CEP - secondary branch
EG - Zoology
CEP - another secondary branch
—
10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
10613 - Zoology
10614 - Behavioral sciences biology
10615 - Ornithology
10616 - Entomology
30101 - Human genetics
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
The project brought original data on structure, molecular differentiation and composition of the W chromosome in Lepidoptera. Results obtained represent a significant progress in understanding the W chromosome evolution and its role in sex determination.
Solution timeline
Realization period - beginning
Jan 1, 2003
Realization period - end
Jan 1, 2005
Project status
U - Finished project
Latest support payment
—
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP06-AV0-IA-U/04:2
Data delivery date
Sep 26, 2007
Finance
Total approved costs
2,261 thou. CZK
Public financial support
1,338 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
2 261 CZK thou.
Public support
1 338 CZK thou.
59%
Provider
Academy of Sciences of the Czech Republic
CEP
EB - Genetics and molecular biology
Solution period
01. 01. 2003 - 01. 01. 2005