Study of regulation of the invertase encoding gene expression in the yeast Schwanniomyces occidentalis

Provider

Academy of Sciences of the Czech Republic

Programme

Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the Academy of Sciences of the Czech Republic

Call for proposals

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Main participants

Mikrobiologický ústav AV ČR, v. v. i.

Contest type

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Contract ID

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Project name in Czech

Studium regulace exprese genu kódujícího invertasu v kvasince Schwanniomyces occidentalis

Annotation in Czech

Cílem předkládaného projektu je objasnění roce 5 Nukleotidové sekvence zúčastněné v regulaci exprese genu budou indntifikovány deleční analýzou promotorové oblasti genu INV. Společně se sekvenování DNA a počítačovou analýzou bude zjištěna jejich přesná lokalizace ve studované oblasti. Podrobná charakterizace promotorové oblasti genu INV. tj. nalezení sekvencí zodpovědných za derepresi, represi i případnou konstitutivní syntézu invertasy, je nezbytná pro další používání této regulační oblasti při konstrukci expresních vektorů pro S. occidentalis a přispěje také k poznání mechanismů katabolické represe v buňkách S. occidentalis.

R&D category

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CEP classification - main branch

EB - Genetics and molecular biology

CEP - secondary branch

EI - Biotechnology and bionics

CEP - another secondary branch

EE - Microbiology, virology

OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)

10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10606 - Microbiology<br>10607 - Virology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>20801 - Environmental biotechnology<br>20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management<br>20803 - Environmental biotechnology related ethics<br>20901 - Industrial biotechnology<br>20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation<br>20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials<br>30101 - Human genetics<br>30401 - Health-related biotechnology<br>30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)<br>30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])<br>30404 - Biomaterials (as related to medical implants, devices, sensors)<br>30405 - Medical biotechnology related ethics<br>40401 - Agricultural biotechnology and food biotechnology<br>40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming<br>40403 - Agricultural biotechnology related ethics

Realization period - beginning

Jan 1, 1997

Realization period - end

Jan 1, 1997

Project status

J - One year project

Latest support payment

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Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data delivery code

CEP/1997/AV0/AV07IA/V/9:4

Data delivery date

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Total approved costs

196 thou. CZK

Public financial support

196 thou. CZK

Other public sources

0 thou. CZK

Non public and foreign sources

0 thou. CZK