Insight into the transcriptional regulation of the crosstalk between plant hormones and the stress response in fruits
Public support
Provider
Ministry of Education, Youth and Sports
Programme
INTER-EXCELLENCE
Call for proposals
SMSM2020LTC01
Main participants
Masarykova univerzita / Středoevropský technologický institut
Contest type
VS - Public tender
Contract ID
MSMT-21080/2020-4
Alternative language
Project name in Czech
Insight into the transcriptional regulation of the crosstalk between plant hormones and the stress response in fruits
Annotation in Czech
Post-harvest fruit management is an essential process for the storage and distribution of fruits and vegetables after their harvest while maintaining their nutritional values, vitamins, and antioxidants content. Up to 14% of fruit and vegetable production losses are the results of post-harvest fruit and vegetable waste. It has been recently proposed that fruit ripening following harvest results of low oxygen and oxidative stress, and increased levels of ethylene, the ripening hormone in climacteric fruits. The COST Action RoxyCOST CA18210, “Oxygen sensing, a novel mean for biology and technology of fruit quality”, aims at investigating the crosstalk between low-oxygen sensing, oxidative stress and ethylene signalling for climacteric fruit ripening with the ultimate goal to propose solutions to extend the post-harvesting period of such fruits. For this purpose, it brings together European scientists with diverse expertise to deliver solutions to this issue. This INTER-COST project will help to solve the problematics of the RoxyCOST Working Group 2 “Deciphering the mechanisms of low oxygen sensing in fruit crops” by developing a hairy root culture system in fruit crop species tomato and almond x peach hybrid. The methodology relies on the injection of a plant pathogen Agrobacterium containing a root-inducing (Ri) plasmid into the stem of the seedlings. In close collaboration with two COST partners, the teams of J. PIRRELLO (Toulouse, France), chair of the action, and Maria RUBIO CABETAS (Zaragoza, Spain), we will adapt this method to tomato and almond x peach hybrid to functionally analyse transcription factors and their selected targets in the ethylene and low oxygen-sensing pathways. This project will increase the technical expertise, knowledge, and the international collaborative network of the Czech research team, led by the applicant, H. Robert Boisivon, at CEITEC Masaryk University, Brno.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10611 - Plant sciences, botany
OECD FORD - secondary branch
10605 - Developmental biology
OECD FORD - another secondary branch
—
CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
EB - Genetics and molecular biology<br>EF - Botany
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
The project's outputs advanced the understanding of plant tissue cultures by unveiling new insights. We developed a protocol for inducing hairy roots in tomato, almond, and diverse Brassicaceae species. Employing CRISPR/Cas techniques, we introduced mutations in genes linked to oxidative stress in almond roots. The project findings were published in international peer-reviewed journals.
Solution timeline
Realization period - beginning
Jun 1, 2020
Realization period - end
Sep 30, 2023
Project status
U - Finished project
Latest support payment
Feb 17, 2023
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP24-MSM-LT-U
Data delivery date
Jul 1, 2024
Finance
Total approved costs
3,930 thou. CZK
Public financial support
3,930 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK