Enzymatic preparation of glyconanomaterials

Provider

Ministry of Education, Youth and Sports

Programme

INTER-EXCELLENCE

Call for proposals

SMSM2020LTC01

Main participants

Mikrobiologický ústav AV ČR, v. v. i.

Contest type

VS - Public tender

Contract ID

MSMT-21080/2020-36

Project name in Czech

Enzymatic preparation of glyconanomaterials

Annotation in Czech

Major objective of this project proposal is to prepare rhamnose and rutinose containing glycoconjugates and multivalent structures with the use of advanced chemoenzymatic methods and bring these compounds into the applications in treatment of dermatological problems and diabetic ulcer complications. Rhamnosyl-rich oligo- and polysaccharides proved to be active in inhibiting most of the harmful effects of AGE-products - typically wrinkle formation and they demonstrated a potential to revert or diminish their negative consequences. This effect may be caused by the interaction with the hypothetic α-rhamnose-selective lectins e.g. in keratinocytes. We will prepare new multivalent rhamnose carrying structures based on the presentation of rutinosyl moieties (α-L-Rha(16)-β-D-Glc), which can be attached onto various spacers using enzymatic transglycosylation with recently discovered enzyme rutinosidase. These rhamnosyl neoglycoconjugates will be used for rhamnose lectin search and tested in biological assays for inhibitory activity towards AGE-mediated pathologies.

R&D category

ZV - Basic research

OECD FORD - main branch

10608 - Biochemistry and molecular biology

OECD FORD - secondary branch

30104 - Pharmacology and pharmacy

OECD FORD - another secondary branch

30401 - Health-related biotechnology

CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)

CE - Biochemistry<br>EB - Genetics and molecular biology<br>EI - Biotechnology and bionics<br>FR - Pharmacology and apothecary chemistry

Provider evaluation

V - Vynikající výsledky projektu (s mezinárodním významem atd.)

Project results evaluation

The dual substrate specificity of Aspergillus niger rutinosidase and its substrate tunnel were discovered. This rutinosidase is capable of glycosylating inorganic azide. The native enzyme formed the beta-anomer and the mutant formed the alpha-anomer. Rutinose-based rhamnose derivatives interact with rhamnose recognising lectins and inhibit aging-related effects in the skin (anti-aging activity).

Realization period - beginning

Jun 1, 2020

Realization period - end

Oct 3, 2023

Project status

U - Finished project

Latest support payment

Feb 17, 2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data delivery code

CEP24-MSM-LT-U

Data delivery date

Jul 1, 2024

Total approved costs

3,858 thou. CZK

Public financial support

3,858 thou. CZK

Other public sources

0 thou. CZK

Non public and foreign sources

0 thou. CZK