Ecology of cereal viruses and development of microarray chip and Real-time PCR platforms for identification and quantification of cereal viruses in natural and agricultural ecosystems.
Public support
Provider
Ministry of Education, Youth and Sports
Programme
KONTAKT
Call for proposals
KONTAKT 8 (SMSM2010ME5)
Main participants
—
Contest type
VS - Public tender
Contract ID
2062/2011-320
Alternative language
Project name in Czech
Ekologie obilných virů a vývoj microarray čipu a Real-time PCR metod pro identifikaci a kvantifikaci těchto virů v přirozených a zemědělských ekosystémech
Annotation in Czech
Vyvinout metody microarray pro multiplexní diagnostiku virových patogenů Poaceae a kvantifikace těchto patogenů pomocí qPCR umožňující upřesnit distribuci, etiologii a zdroje epidemie těchto patogenů v řízených a neřízených ekosystémech.
Scientific branches
R&D category
AP - Applied research
CEP classification - main branch
GF - Diseases, pests, weeds and plant protection
CEP - secondary branch
EE - Microbiology, virology
CEP - another secondary branch
EB - Genetics and molecular biology
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10606 - Microbiology<br>10607 - Virology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>30101 - Human genetics<br>40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
The specific oligo-probes for Microarray methods were developed for the detection of viruses of cereals and grasses. The genus Luteovirus, Mastrevirus, Tritimovirus, Bymovirus and their species and strains were detected by microarray. Real-time (RT) qPCRassays were developed for detection and quantification of DNA and RNA viruses infecting cereals and grasses.
Solution timeline
Realization period - beginning
May 1, 2010
Realization period - end
Dec 31, 2012
Project status
U - Finished project
Latest support payment
Feb 16, 2012
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP13-MSM-ME-U/01:1
Data delivery date
Jun 28, 2013
Finance
Total approved costs
1,950 thou. CZK
Public financial support
1,950 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK