The role of post-translational modification in DNA repair and recombination: Meiosis, SUMOylation and Zip3 protein
Public support
Provider
Ministry of Education, Youth and Sports
Programme
KONTAKT
Call for proposals
KONTAKT 8 (SMSM2010ME5)
Main participants
—
Contest type
VS - Public tender
Contract ID
2062/2011-320
Alternative language
Project name in Czech
Vliv post-translačních modifikací na DNA opravu a rekombinaci.
Annotation in Czech
Cílem projektu je pochopení funkce SUMO proteinu v procesu homologní rekombinace, který je nezbytnou součástí meiotického dělení buněk umožňujícího pohlavní rozmnožování a zároveň je jedním z klíčových mechanismů zajišťujících opravu poškozené DNA.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
CE - Biochemistry
CEP - secondary branch
EB - Genetics and molecular biology
CEP - another secondary branch
—
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>30101 - Human genetics
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
During characterization of the role of Lif1 sumoylation we have identified main conjugation lysine and tested the effect of DNA and E3 ligases on its sumoylation. Using 2-hybrid system we have shown the effect of sumoylation on the oligomerization of Lif1 protein and our data indicate that sumoylation down-regulates the efficiency of end joining and represents a new regulatory element.
Solution timeline
Realization period - beginning
May 1, 2010
Realization period - end
Dec 31, 2012
Project status
U - Finished project
Latest support payment
Feb 16, 2012
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP13-MSM-ME-U/01:1
Data delivery date
Jun 28, 2013
Finance
Total approved costs
1,632 thou. CZK
Public financial support
1,632 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK