Genetic variability of pro- and anti-inflammatory cytokines in pathogenesis of rheumatoid arthritis
Project goals
Assesment of genetic variability of genes in cytokine net in respect to rheumatoid arthritis and its intermediate phenotype, inclusive of the analysis of selected cytokines plasma levels. For detection of pholymorphisms in promoter and introne regions, PCR and RFLP methods will be used. New polymorphisms in regulatory regions of IL-8, IL-13 and IL-15 genes will be detected by heteroduplex analysis and SSCP. The protein levels of cytokines will be measured by enzyme-amplified chemiluminiscence and ELISAmethod. The "case-control" (association) study will be used to assess the relation between genotype and disease. The significance of the differences will be calculated by chi-square test and Fisher's exact test. Odds ratio (OR) with 95% confidence interval will be used to estimate the relative risks.
Keywords
Public support
Provider
Ministry of Health
Programme
Branch programm of research of the Ministry of Health
Call for proposals
VaV pro Ministerstvo zdravotnictví 1 (SMZ02004NR)
Main participants
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Contest type
VS - Public tender
Contract ID
—
Alternative language
Project name in Czech
Genetická variabilita pro- a proti-zánětlivých cytokinů v patogenezi revmatoidní artritidy
Annotation in Czech
Zhodnocení genetické variability genů cytokinové sítě vzhledem k revmatoidní artritidě a jejímu intermediárnímu fenotypu, včetně analýzy plazmatických hladin vybraných cytokinů. Metod PCR a RFLP bude využito ke stanovení promotorových a intronových polymorfismů. Nové polymorfismy budou v regulačních oblastech genů pro IL-8, IL-13 a IL-15 detekovány pomocí heteroduplexní analýzy a SSCP. Proteinové hladiny vybraných cytokinů budou hodnoceny pomocí enzymaticky zesílené chemiluminiscence a ELISA metody. K vyjádření vtahu mezi genotypy a onemocněním bude využita tzv. "case-control" studie (asociační studie - "případ-kontroly"). Významnost rozdílů bude hodnocena pomocí chi-square testu a Fisher exact testu. Odds ratio (OR) s 95% konfidenčním intervalem budevyužito ke stanovení relativního rizika ve vztahu k RA.
Scientific branches
R&D category
NV - Nonindustrial research (Applied research excluded Industrial research)
CEP classification - main branch
FE - Other fields of internal medicine
CEP - secondary branch
EB - Genetics and molecular biology
CEP - another secondary branch
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10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
30101 - Human genetics
30217 - Urology and nephrology
30218 - General and internal medicine
30219 - Gastroenterology and hepatology
30220 - Andrology
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
The presence of GG genotype in promoter region of 308TNF alpha is associated with more erosive and severe RA. This was published in a journal with IF. Besides, the relations to polymorphism of gene for IL-13, IL-15 were described.
Solution timeline
Realization period - beginning
Jan 1, 2004
Realization period - end
Jan 1, 2006
Project status
U - Finished project
Latest support payment
—
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP07-MZ0-NR-U/06:2
Data delivery date
Aug 8, 2007
Finance
Total approved costs
2,490 thou. CZK
Public financial support
2,467 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
13 thou. CZK
Basic information
Recognised costs
2 490 CZK thou.
Public support
2 467 CZK thou.
99%
Provider
Ministry of Health
CEP
FE - Other fields of internal medicine
Solution period
01. 01. 2004 - 01. 01. 2006