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NR7989

Targeted gene silencing using siRNAs in primary cell cultures of CML patients and leukemic cell lines (K562, MOLM-7, KU-812)

Public support

  • Provider

    Ministry of Health

  • Programme

    Branch programm of research of the Ministry of Health

  • Call for proposals

    VaV pro Ministerstvo zdravotnictví 1 (SMZ02004NR)

  • Main participants

  • Contest type

    VS - Public tender

  • Contract ID

Alternative language

  • Project name in Czech

    Cílené tlumení genové aktivity pomocí siRNA v primárních buněčných kulturách pacientů s CML a leukemických buněčných liniích (K562, MOLM-7, KU-812)

  • Annotation in Czech

    V projektu budou využity siRNA pro potlačení genové exprese genů MMP8, MMP9, PCNA, MAPK p38, Bmi-1, BCR/ABL u leukemických buněčných liních odvozených od CML pacientů a u primárních kultur získaných kultivací leukocytů z periferní krve CML pacientů.

Scientific branches

  • R&D category

    NV - Nonindustrial research (Applied research excluded Industrial research)

  • CEP classification - main branch

    FD - Oncology and haematology

  • CEP - secondary branch

    EB - Genetics and molecular biology

  • CEP - another secondary branch

  • OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)

    10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>30101 - Human genetics<br>30204 - Oncology<br>30205 - Hematology

Completed project evaluation

  • Provider evaluation

    V - Vynikající výsledky projektu (s mezinárodním významem atd.)

  • Project results evaluation

    Protocols for siRNA use were optimized and siRNA for inhibition of PCNA, MMP8/9, p38, JNK2, Bcr/Abl and Bmi-1 genes were prepared. siRNA use in functional studies of these genes was explored.

Solution timeline

  • Realization period - beginning

    Jan 1, 2004

  • Realization period - end

    Jan 1, 2006

  • Project status

    U - Finished project

  • Latest support payment

Data delivery to CEP

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

  • Data delivery code

    CEP07-MZ0-NR-U/06:2

  • Data delivery date

    Aug 8, 2007

Finance

  • Total approved costs

    3,699 thou. CZK

  • Public financial support

    3,291 thou. CZK

  • Other public sources

    247 thou. CZK

  • Non public and foreign sources

    0 thou. CZK