The role of apoptosis in the ontogenesis and stress responses of higher plant cells and organs
Project goals
The phenomenon of programmed cell death (PCD, apoptosis) and its role in plant morphogenesis and stress responses will be studied by means of different model systems: plant cell lines, hydroponically cultures seedlings and the plants of natural communities. The applicability of various standard methods of PCD detection on the molecular, biochemical and metabolical level for the plant material will be verified . The role of PCD in the bud morphogenesis (conifers) and stem vessel compounds differentiation(wetland plants, cereales) will be investigated. The apoptosis-like effect of abiotic stress (including PCD-inducible role of heavy metals) on the plant cell lines will be characterized and their possibility to be used as bioindicators for systematic screening of ecotoxic factors will be evaluated.
Keywords
programmed cell deathapoptosisabiotic stresscell linesplant morphogenesisecotoxicitymolecular markersheavy metalsconiferswetland plants
Public support
Provider
Ministry of Education, Youth and Sports
Programme
COST
Call for proposals
COST 1 (SMSM200132004)
Main participants
Univerzita Karlova / Přírodovědecká fakulta
Contest type
VS - Public tender
Contract ID
—
Alternative language
Project name in Czech
Úloha apoptózy v ontogenezi a v stresových reakcích rostlinných buněk a orgánů.
Annotation in Czech
Úloha programované buněčné smrti (PCD, apoptózy) v morfogenezi rostlin a v jejich reakci na různé typy stresu bude studována s pomocí několika modelových objektů: buněčných linií, hydroponicky pěstovaných rostlin i rostlin z přirozených společenstev. Budou ověřeny a porovnány různé techniky detekce PCD na molekulární, metabolické i strukturální úrovni. Bude sledována role PCD v diferenciaci pupenů u jehličnanů, cévních svazků u mokřadních rostlin . Bude charakterizován účinek těžkých kovů a dalších ekotoxických faktorů na iniciaci PCD a ověřena možnost využití buněčných linií jako standardních bioindikátorů .
Scientific branches
R&D category
NV - Nonindustrial research (Applied research excluded Industrial research)
CEP classification - main branch
ED - Physiology
CEP - secondary branch
EB - Genetics and molecular biology
CEP - another secondary branch
EH - Ecology - communities
10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
10618 - Ecology
10619 - Biodiversity conservation
30101 - Human genetics
30105 - Physiology (including cytology)
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
Unique transgenic lines for PCD study and a classification scale of molecular, cytological and biochemical symptoms of PCD in plant cells was prepared. Induction of PCD was studied in response to abiotic and biotic stressors and cytokinins. 2 publication
Solution timeline
Realization period - beginning
Jan 1, 2000
Realization period - end
Jan 1, 2005
Project status
U - Finished project
Latest support payment
—
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP06-MSM-OC-U/04:2
Data delivery date
May 28, 2008
Finance
Total approved costs
6,350 thou. CZK
Public financial support
1,890 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
4,400 thou. CZK
Recognised costs
6 350 CZK thou.
Public support
1 890 CZK thou.
0%
Provider
Ministry of Education, Youth and Sports
CEP
ED - Physiology
Solution period
01. 01. 2000 - 01. 01. 2005