Importance of nucleoprotein complexes for the regulation of the male gametophyte development
Public support
Provider
Ministry of Education, Youth and Sports
Programme
COST
Call for proposals
COST 6 (SMSM2008OC3)
Main participants
—
Contest type
VS - Public tender
Contract ID
1146/2010-32
Alternative language
Project name in Czech
Nukleoproteinové komplexy a jejich úloha při regulaci vývoje samčího gametofytu
Annotation in Czech
Proteomická, funkční a biochemická charakterizace specifických ribonukleoproteinových komplexů (EPP), které obsahují skladovanou mRNA a jsou v samčím gametofytu klíčovou složkou systému regulace genové exprese na úrovni translace
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
ED - Physiology
CEP - secondary branch
EB - Genetics and molecular biology
CEP - another secondary branch
EF - Botany
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>10611 - Plant sciences, botany<br>10612 - Mycology<br>30101 - Human genetics<br>30105 - Physiology (including cytology)
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
We have implemented and used method for the isolation of EPP particles. Their subsequent proteomic analysis revealed their protein composition. We further implemented and optimized method for the phosphoprotein enrichment. The subsequent MS analysis ledto the identification of 15 proteins differentially phoshorylated between mature pollen and tubes. We identified mRNA populations within EPPs.
Solution timeline
Realization period - beginning
Jan 1, 2008
Realization period - end
Mar 31, 2011
Project status
U - Finished project
Latest support payment
Feb 22, 2011
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP12-MSM-OC-U/01:1
Data delivery date
Jul 12, 2012
Finance
Total approved costs
2,430 thou. CZK
Public financial support
1,625 thou. CZK
Other public sources
805 thou. CZK
Non public and foreign sources
0 thou. CZK