Tyrophagus putrescentiae as a vector of mycotoxin-producing fungi in stored fodder barley
Project goals
In this project we will test the hypothesis that the mites can transfer fungi to the stored human food and animal feed and thus cause mycotoxin contamination. To test the hypothesis, the milestones were set as follows:(1) The construction and optimization of PCR markers for identification of mycotoxin producing fungal strains or mycotoxin biosynthetic genes. (2) The transfer of fungi via mites into sterile grain in the microcosms. (3) The transfer of fungi via mites into grain with the developed fungalcommunity in the microcosms. In the both designs we will observe the contents of mycotoxins in the microcosms in relation to the number of transferring mites. The outputs of the project are the primers and optimized PCR methods and 2 papers in scientificjournals.
Keywords
Public support
Provider
Ministry of Education, Youth and Sports
Programme
COST
Call for proposals
COST 8 (SMSM2010OC5)
Main participants
—
Contest type
VS - Public tender
Contract ID
1081/2011-320
Alternative language
Project name in Czech
Roztoč Tyrophagus putrescentiae jako přenašeč producentů mykotoxinů ve skladovaném ječmeni
Annotation in Czech
Prokázat transport mikroskopických hub rodu Fusarium a Penicillium, které produkují mykotoxiny, na povrchu a v exkrementech roztoče Tyrophagus putrescentiae v ječmeni. Transport bude sledován v mikrokosmových experimentech.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
GF - Diseases, pests, weeds and plant protection
CEP - secondary branch
GH - Nutrition of farm animals
CEP - another secondary branch
GM - Food industry
21101 - Food and beverages
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)
40202 - Pets
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
We have observed the transport of Fusarium fungi via mite Tyrophagus putrescentiae. As the markers we observe the presence of Fusarium poae DNA in the samples infested by F.poae preincubated mites and the increase of deoxinivalenol concentration in thesesamples. The output of the project is paper in IF journal and e- book, the paper submitted to IF journal.
Solution timeline
Realization period - beginning
May 1, 2010
Realization period - end
Dec 31, 2012
Project status
U - Finished project
Latest support payment
Feb 27, 2012
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP13-MSM-OC-U/01:1
Data delivery date
Jun 28, 2013
Finance
Total approved costs
1,324 thou. CZK
Public financial support
1,250 thou. CZK
Other public sources
74 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
1 324 CZK thou.
Public support
1 250 CZK thou.
94%
Provider
Ministry of Education, Youth and Sports
CEP
GF - Diseases, pests, weeds and plant protection
Solution period
01. 05. 2010 - 31. 12. 2012