Species identification in routine casework samples using the SPInDel kit

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00007064%3AK01__%2F19%3AN0000048" target="_blank" >RIV/00007064:K01__/19:N0000048 - isvavai.cz</a>

Result on the web

<a href="https://www.fsigeneticssup.com/article/S1875-1768(19)30304-X/fulltext" target="_blank" >https://www.fsigeneticssup.com/article/S1875-1768(19)30304-X/fulltext</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fsigss.2019.09.070" target="_blank" >10.1016/j.fsigss.2019.09.070</a>

Result language

angličtina

Original language name

Species identification in routine casework samples using the SPInDel kit

Original language description

The identification of species in casework samples is of fundamental importance for forensic investigations. Laboratories are increasingly compelled to provide accurate and fast identifications in trace materials left on crime scenes, wildlife poaching, illegal trade of protected species, fraudulent food products cases, etc. However, the field of nonhuman forensic genetics is still working on the standardization of typing methods and practices. Here we describe the successful implementation of the Species Identification by Insertions/Deletions (SPInDel) method in routine casework analyses in 11 laboratories worldwide. The SPInDel was developed to detect human DNA, at the same time that identifies common animal species. The fragment size analysis of six mtDNA regions allows identification in suboptimal DNA samples, including mixtures, with no need for sequencing. The samples were collected from 2013 to 2018 and included hair, blood, meat, saliva, faeces, bones, etc. The SPInDel kit successfully identified >95% of the samples, being dog, human and pig the most frequently detected species. The six SPInDel loci were successfully amplified in mixtures and degraded samples (river water, sand, stains in clothes, etc.). Interestingly, several species that were not originally targeted by SPInDel primers were also identified (e.g., red fox, brown bear, fallow deer and red deer). In conclusion, the SPInDel kit was successfully used in crime scene investigations (often involving human DNA detection) and in cases of poaching, environmental contamination and food fraud. It is now becoming a useful tool for the routine analysis of nonhuman DNA samples within the high quality standards of forensic genetics.

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

<a href="/en/project/VI20162020015" target="_blank" >VI20162020015: Introduction of New Methods of Human, Animal and Plant Material Identification in Forensic Practice</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Forensic Science International: Genetics

ISSN

1872-4973

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

2

Pages from-to

180-181

UT code for WoS article

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EID of the result in the Scopus database

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