Protocol for efficient micropropagation of spring gentian and sand jurinea

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00020702%3A_____%2F14%3A%230000817" target="_blank" >RIV/00020702:_____/14:#0000817 - isvavai.cz</a>
Result on the web
<a href="http://www.agriculturejournals.cz/publicFiles/112132.pdf" target="_blank" >http://www.agriculturejournals.cz/publicFiles/112132.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Protocol for efficient micropropagation of spring gentian and sand jurinea
Original language description
Protocols for the in vitro propagation of two endangered plant species in the Czech Republic, Gentiana verna L. and Jurinea cyanoides (L.) Rchb., were established. In G. verna, the induction of organogenesis on vegetative shoots was successful on the basal 6% agar WPM medium with 200 mg.l-1 of glutamine, 200 mg.l-1 of casein hydrolysate, 30 g.l-1 of sucrose, 0.2 mg.l-1 of BAP, and o.1 mg.l-1 of IBA. The multiplication of primary explants was achieved on 6% agar basal MS medium with the same concentrations of substances mentioned above. In J. cyanoides, the induction of organogenesis and multiplication of its vegetative shoots were successful on MS medium with the same concentration of added substances used in G. verna. On the basal MS medium of 1/3 concentration with 3 mg.l-1 of IBA 70-75% rooting efficiency of G. verna microcuttings and 40% rooting efficiency of J. cyanoides microcuttings were reached. The mortality during acclimatization did not exceed 20% for G. verna and 25% for J.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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