Long-term observation of in vitro-derived Malus sylvestris (L.) Mill., the path from the bud to the tree

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00020702%3A_____%2F23%3AN0000093" target="_blank" >RIV/00020702:_____/23:N0000093 - isvavai.cz</a>
Result on the web
<a href="https://www.intechopen.com/chapters/84344" target="_blank" >https://www.intechopen.com/chapters/84344</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5772/intechopen.108359" target="_blank" >10.5772/intechopen.108359</a>

Result language
angličtina
Original language name
Long-term observation of in vitro-derived Malus sylvestris (L.) Mill., the path from the bud to the tree
Original language description
The European wild apple (Malus sylvestris L.), a wild contributor to the domesticated apple, belongs to the endangered species in the Czech Republic. Thus, an efficient protocol was developed for in vitro plantlet regeneration using the post-dormant buds. The highest shoot induction was obtained on MS medium supplemented with 0.5 mg.l−1 BAP, 5 mg.l−1 GA and 0.1 mg.l−1 IBA. Shoot multiplication and elongation took place on the same medium with 0.2 mg.l−1 BAP and 0.1 mg.l−1 IBA. Indole-3-butyric acid at 0.5 mg.l−1 was most effective for rooting. The micropropagated plantlets were successfully acclimatized in greenhouse conditions and were transplanted into soil in forest. Finally, qualitative and quantitative parameters of tissue culture-derived plants were evaluated. Monitoring of in vitro plantings on experimental trials suggests that micropropagated wild apple trees retain the growth characteristics of generative individuals.
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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