SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00020711%3A_____%2F24%3A10155183" target="_blank" >RIV/00020711:_____/24:10155183 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/24:00136188 RIV/00216208:11110/24:10482907
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S2589914724000100" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2589914724000100</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.wroa.2024.100220" target="_blank" >10.1016/j.wroa.2024.100220</a>
Alternative languages
Result language
angličtina
Original language name
SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters
Original language description
This study presents the results of systematic wastewater monitoring of SARS-CoV-2 RNA and basic wastewater parameters from four different wastewater treatment plants (WWTPs) in the Czech Republic over the 2020-2022 epidemic. Two-step reverse-transcription quantitative PCR targeting genes encoding the N and Nsp12 proteins was employed to detect SARS-CoV-2 RNA loading in 420 wastewater samples. The results obtained were used to evaluate the potential of wastewater analysis for describing the epidemiological situation in cities of different sizes and determining temporal differences based on the prevailing SARS-CoV-2 variant. Strong correlations between the number of active and hospitalised COVID-19 cases in each WWTP catchment area and the concentration of SARS-CoV-2 RNA detected in the wastewater clearly demonstrated the suitability of this wastewater-based epidemiological approach for WWTPs of different sizes and characteristics, despite differences in SARS-CoV-2 variant waves, with some WWTPs showing high predictive potential. This study demonstrated on the data from the Czech Republic that targeted systematic monitoring of wastewater provides sufficiently robust data for surveillance of viral loads in sample populations, and thus contributes to preventing the spread of infection and subsequent introduction of appropriate measures.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30303 - Infectious Diseases
Result continuities
Project
<a href="/en/project/VI04000017" target="_blank" >VI04000017: Wastewater monitoring used as an early warning tool for epidemic outbreak</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Water Research X
ISSN
2589-9147
e-ISSN
2589-9147
Volume of the periodical
23
Issue of the periodical within the volume
March 28, 2024
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
1-8
UT code for WoS article
001234113000001
EID of the result in the Scopus database
2-s2.0-85189934317