Effect of short- and long-term high-fat feeding on autophagy flux and lysosomal activity in rat liver

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F12%3A00056075" target="_blank" >RIV/00023001:_____/12:00056075 - isvavai.cz</a>
Result on the web
<a href="http://www.biomed.cas.cz/physiolres/pdf/61%20Suppl%202/61_S67.pdf" target="_blank" >http://www.biomed.cas.cz/physiolres/pdf/61%20Suppl%202/61_S67.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Effect of short- and long-term high-fat feeding on autophagy flux and lysosomal activity in rat liver
Original language description
Autophagy-lysosomal pathway is a cellular mechanism ensuring degradation of various macromolecules like proteins or triacylglycerols (TAG). Its disruption is related to many pathological states, including liver steatosis. We compared the effect of short-and long-established steatosis on the intensity of autophagy-lysosomal pathway in rat liver. The experiments were carried out on 3-month old Wistar rats fed standard (SD) or high-fat diet for 2 (HF-2) or 10 (HF-10) weeks. HF diet administered animals accumulated an increased amount of TAG in the liver (HF-2 -> HF-10). Autophagy flux was up-regulated in HF-2 group but nearly inhibited after 10 weeks of HF administration. The expression of autophagy related genes was up-regulated in HF-2 but normal in HF-10. In contrast, total activities of two lysosomal enzymes, lysosomal lipase (LAL) and acid phosphatase, were unaffected in HF-2 but significantly increased in HF-10 groups. mRNA expression of lysosomal enzymes was not affected by the die
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FB - Endocrinology, diabetology, metabolism, nutrition
OECD FORD branch
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Project
<a href="/en/project/GAP301%2F11%2F2418" target="_blank" >GAP301/11/2418: Using 13C and proton MR spectroscopy to study the role of fatty acid transport and accumulation in tissues in the pathogenesis of insulin resistance</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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