Silybin affects the liver microsomal CYP2C6 in HHTg rats

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F13%3A00058739" target="_blank" >RIV/00023001:_____/13:00058739 - isvavai.cz</a>

Alternative codes found

RIV/00098892:_____/13:#0000416

Result on the web

<a href="http://biomed.papers.upol.cz/incpdfs/pri-990000-0500_10_031.pdf" target="_blank" >http://biomed.papers.upol.cz/incpdfs/pri-990000-0500_10_031.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Silybin affects the liver microsomal CYP2C6 in HHTg rats

Original language description

Aim. This study aims to investigate the effect of silybin on liver expression and activity of rat CYP2C6 under mild liver steatosis. This cytochrome P450 enzyme is considered to be a counterpart of human CYP2C9, which metabolizes commonly prescribed drugs, such as ibuprofen, diclofenac, or warfarin. Methods. Male hereditary hypertriglyceridemic rats (accepted model of metabolic syndrome) were fed: 1) standard laboratory diet (STD), 2) high cholesterol diet (HCD = STD + 1% of cholesterol w/w + 10% of lard fat w/w), 3) high cholesterol diet with silybin (0.5% w/w) for 21 days. Expression of cytochrome P450 2C6 was measured in liver using real-time PCR (at mRNA level) and Western blotting (at protein level). Formation of diclofenac metabolite (typical marker substrate of CYP2C6 enzyme activity) was analyzed using HPLC with UV detection. Results. Silybin in hereditary hypertriglyceridemic rats on HCD diet significantly increased activity of CYP2C6 and its expression on mRNA level. Expressi

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FB - Endocrinology, diabetology, metabolism, nutrition

OECD FORD branch

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Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical papers

ISSN

1213-8118

e-ISSN

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Volume of the periodical

157

Issue of the periodical within the volume

suppl. 1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

"S60"-"S64"

UT code for WoS article

000324728600004

EID of the result in the Scopus database

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