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HNF-4 alpha regulates expression of human ornithin carbamoyltransferase through interaction with two positive cis-Aacting regulatory elements located in the proximal promoter

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F14%3A00059063" target="_blank" >RIV/00023001:_____/14:00059063 - isvavai.cz</a>

  • Result on the web

    <a href="http://fb.cuni.cz/file/5725/fb2014a0017.pdf" target="_blank" >http://fb.cuni.cz/file/5725/fb2014a0017.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    HNF-4 alpha regulates expression of human ornithin carbamoyltransferase through interaction with two positive cis-Aacting regulatory elements located in the proximal promoter

  • Original language description

    OTC encodes ornithine carbamoyltransferase, mitochondrial matrix enzyme involved in the synthesis of urea. The tissue-specific expression of OTC in the liver and intestine is dependent on the interaction of OTC promoter with an upstream enhancer. HNF-4 and C/EBP beta are crucial for this interaction in the rat and mouse. In the present study we focused on characterization of elements involved in the regulation of OTC transcription in human. Using a set of 5'-deleted promoter mutants in a reporter assaywe identified two positive cis-acting regulatory elements located at c.-105 and c.-136 within the human OTC promoter. Both are essential for the transcriptional activity of the promoter itself and for the interaction with the enhancer. Protein binding atthe corresponding sites was confirmed by DNase I footprinting. Electromobility shift assay with a specific competitor and anti-HNF-4 alpha antibody identified the DNA-protein binding sites as HNF-4 alpha recognition motifs. A third IINF-

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    FE - Other fields of internal medicine

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Folia biologica (Praha)

  • ISSN

    0015-5500

  • e-ISSN

  • Volume of the periodical

    60

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    11

  • Pages from-to

    133-143

  • UT code for WoS article

    000338479400005

  • EID of the result in the Scopus database