HNF-4 alpha regulates expression of human ornithin carbamoyltransferase through interaction with two positive cis-Aacting regulatory elements located in the proximal promoter

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F14%3A00059063" target="_blank" >RIV/00023001:_____/14:00059063 - isvavai.cz</a>

Result on the web

<a href="http://fb.cuni.cz/file/5725/fb2014a0017.pdf" target="_blank" >http://fb.cuni.cz/file/5725/fb2014a0017.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

HNF-4 alpha regulates expression of human ornithin carbamoyltransferase through interaction with two positive cis-Aacting regulatory elements located in the proximal promoter

Original language description

OTC encodes ornithine carbamoyltransferase, mitochondrial matrix enzyme involved in the synthesis of urea. The tissue-specific expression of OTC in the liver and intestine is dependent on the interaction of OTC promoter with an upstream enhancer. HNF-4 and C/EBP beta are crucial for this interaction in the rat and mouse. In the present study we focused on characterization of elements involved in the regulation of OTC transcription in human. Using a set of 5'-deleted promoter mutants in a reporter assaywe identified two positive cis-acting regulatory elements located at c.-105 and c.-136 within the human OTC promoter. Both are essential for the transcriptional activity of the promoter itself and for the interaction with the enhancer. Protein binding atthe corresponding sites was confirmed by DNase I footprinting. Electromobility shift assay with a specific competitor and anti-HNF-4 alpha antibody identified the DNA-protein binding sites as HNF-4 alpha recognition motifs. A third IINF-

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FE - Other fields of internal medicine

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia biologica (Praha)

ISSN

0015-5500

e-ISSN

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Volume of the periodical

60

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

133-143

UT code for WoS article

000338479400005

EID of the result in the Scopus database

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