Molecular diagnostics identifies risks for graft dysfunction despite borderline histologic changes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F15%3A00059562" target="_blank" >RIV/00023001:_____/15:00059562 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/15:10306868 RIV/00216208:11140/15:10306868 RIV/00064190:_____/15:#0001125 RIV/00023736:_____/15:00011382
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S215717161532267X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S215717161532267X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/ki.2015.211" target="_blank" >10.1038/ki.2015.211</a>
Alternative languages
Result language
angličtina
Original language name
Molecular diagnostics identifies risks for graft dysfunction despite borderline histologic changes
Original language description
The significance of borderline changes in kidney allograft biopsies is widely debated. To help resolve this, we studied differences in intrarenal gene expression patterns between early clinical and 3-month protocol biopsies, all of which had borderline histologic changes. The gene expression profiles in training set of patients by microarray analysis and data were validated in a larger cohort using RT-qPCR. There was greater expression of immunity-and inflammation-related genes in the early clinical biopsies compared to the 3-month protocol biopsies with borderline changes. In early clinically manifested borderline changes, graft deterioration within 24 months due to chronic rejection was associated with increased activation of immune, defense, and inflammatory processes. Regression modeling identified higher donor age and expression of macrophage receptor CLEC5A as risk factors for progression. In the 3-month protocol biopsies with borderline changes, graft dysfunction was associated with increased expression of fibrinogen complex transcripts. The discrimination power of fibrinogen was confirmed by cross-validation on two independent cohorts. Thus, our study highlights variations in gene expression between clinical and subclinical borderline changes despite similar histological findings. The data also support a recommendation for frequent patient monitoring, especially in those with borderline changes who received grafts from older donors.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30213 - Transplantation
Result continuities
Project
<a href="/en/project/7E13020" target="_blank" >7E13020: Personalized minimization of immunosuppression after solid organ transplantation by biomarker-driven stratification of patients to improve long-term outcome and health-economic data of transplantation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Kidney international
ISSN
0085-2538
e-ISSN
—
Volume of the periodical
88
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
785-795
UT code for WoS article
000362219600019
EID of the result in the Scopus database
2-s2.0-84942889787