Identification of gene transcripts implicated in peritoneal membrane alterations

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F16%3A00060147" target="_blank" >RIV/00023001:_____/16:00060147 - isvavai.cz</a>

Result on the web

<a href="http://www.pdiconnect.com/content/36/6/606.long" target="_blank" >http://www.pdiconnect.com/content/36/6/606.long</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3747/pdi.2015.00094" target="_blank" >10.3747/pdi.2015.00094</a>

Result language

angličtina

Original language name

Identification of gene transcripts implicated in peritoneal membrane alterations

Original language description

BACKGROUND: Permanent stimulation of the peritoneum during peritoneal dialysis (PD) is likely to result in increased expression of genes encoding proteins involved in inflammation and tissue remodeling. Peritoneal fibrosis and neoangiogenesis may develop. ♦ OBJECTIVE: To assess highly expressed genes potentially in volved in peritoneal alterations during PD treatment using an animal model. ♦ METHODS: A PD catheter was implanted in 36 male Wistar rats after 70% nephrectomy. The rats were divided into 3 groups, exposed to dialysis solution for 8 weeks, and sacrificed 2 weeks later. Group B was exposed to a buffer, group D was exposed to a 3.86% glucose-based dialysis solution, and in group D+H, a second hit of intraperitoneal blood on top of the dialysis solution was given to induce the development of peritoneal sclerosis. Before sacrifice, peritoneal function was assessed. Omental tissue was obtained for analysis of gene expression using RT-qPCR. ♦ RESULTS: Fibrosis scores, vessel counts, and peritoneal function parameters were not different between the groups. Genes involved in the transforming growth factor beta signaling pathway, cell proliferation, angiogenesis, and inflammation were more expressed (p < 0.05) in the D+H group. Almost no differences were found between the control groups. We identified 4 genes that were related to peritoneal transport. ♦ CONCLUSION: Already a mid-term peritoneal exposure, when no microscopical and functional alterations are present, provokes activation of gene pathways of cell proliferation, fibrosis, neoangiogenesis, and inflammation.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FE - Other fields of internal medicine

OECD FORD branch

—

Project

<a href="/en/project/NV15-26638A" target="_blank" >NV15-26638A: Identification of genes implicated in peritoneal membrane alterations during peritoneal dialysis treatment</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Peritoneal dialysis international

ISSN

0896-8608

e-ISSN

—

Volume of the periodical

36

Issue of the periodical within the volume

6

Country of publishing house

CA - CANADA

Number of pages

8

Pages from-to

606-613

UT code for WoS article

000389655100006

EID of the result in the Scopus database

—