An alteration of lymphocytes subpopulations and immunoglobulins levels in patients with diabetic foot ulcers infected particulary by resistant pathogens

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F16%3A00060167" target="_blank" >RIV/00023001:_____/16:00060167 - isvavai.cz</a>

Result on the web

<a href="https://www.hindawi.com/journals/jdr/2016/2356870/" target="_blank" >https://www.hindawi.com/journals/jdr/2016/2356870/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2016/2356870" target="_blank" >10.1155/2016/2356870</a>

Result language

angličtina

Original language name

An alteration of lymphocytes subpopulations and immunoglobulins levels in patients with diabetic foot ulcers infected particulary by resistant pathogens

Original language description

The aim of our study was to analyse immune abnormalities in patients with chronic infected diabetic foot ulcers (DFUs) especially those infected by resistant microorganisms. Methods. 68 patients treated in our foot clinic for infected chronic DFUs with 34 matched diabetic controls were studied. Patients with infected DFUs were subdivided into two subgroups according to the antibiotic sensitivity of causal pathogen: subgroup S infected by sensitive (n = 50) and subgroup R by resistant pathogens (n = 18). Selected immunological markers were compared between the study groups and subgroups. Results. Patients with infected chronic DFUs had, in comparison with diabetic controls, significantly reduced percentages (p < 0.01) and total numbers of lymphocytes (p < 0.001) involving B lymphocytes (p < 0.01), CD4+ (p < 0.01), and CD8+ T cells (p < 0.01) and their naive and memory effector cells. Higher levels of IgG (p < 0.05) including IgG1 (p < 0.001) and IgG3 (p < 0.05) were found in patients with DFUs compared to diabetic controls. Serum levels of immunoglobulin subclasses IgG2 and IgG3 correlated negatively with metabolic control ( < 0.05). A trend towards an increased frequency of IgG2 deficiency was found in patients with DFUs compared to diabetic controls (22% versus 15%; NS). Subgroup R revealed lower levels of immunoglobulins, especially of IgG4 (p < 0.01) in contrast to patients infected by sensitive bacteria. The innate immunity did not differ significantly between the study groups. Conclusion. Our study showed changes mainly in the adaptive immune system represented by low levels of lymphocyte subpopulations and their memory effector cells, and also changes in humoral immunity in patients with DFUs, even those infected by resistant pathogens, in comparison with diabetic controls.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FB - Endocrinology, diabetology, metabolism, nutrition

OECD FORD branch

—

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of diabetes research

ISSN

2314-6745

e-ISSN

—

Volume of the periodical

2016

Issue of the periodical within the volume

2016

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

"Art. ID 2356870"

UT code for WoS article

000390570600001

EID of the result in the Scopus database

—