A Trimodal Imaging Platform for Tracking Viable Transplanted Pancreatic Islets In Vivo: F-19 MR, Fluorescence, and Bioluminescence Imaging
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F19%3A00078034" target="_blank" >RIV/00023001:_____/19:00078034 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/19:10383906 RIV/00216208:11120/19:43917001
Result on the web
<a href="https://link.springer.com/content/pdf/10.1007%2Fs11307-018-1270-3.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007%2Fs11307-018-1270-3.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11307-018-1270-3" target="_blank" >10.1007/s11307-018-1270-3</a>
Alternative languages
Result language
angličtina
Original language name
A Trimodal Imaging Platform for Tracking Viable Transplanted Pancreatic Islets In Vivo: F-19 MR, Fluorescence, and Bioluminescence Imaging
Original language description
PurposeCombining specific and quantitative F-19 magnetic resonance imaging (MRI) with sensitive and convenient optical imaging provides complementary information about the distribution and viability of transplanted pancreatic islet grafts. In this study, pancreatic islets (PIs) were labeled with positively charged multimodal nanoparticles based on poly(lactic-co-glycolic acid) (PLGA-NPs) with encapsulated perfluoro-15-crown-5-ether and the near-infrared fluorescent dye indocyanine green.ProceduresOne thousand and three thousand bioluminescent PIs were transplanted into subcutaneous artificial scaffolds, which served as an alternative transplant site. The grafts were monitored using in vivo F-19 MR, fluorescence, and bioluminescence imaging in healthy rats for 2weeks.ResultsTransplanted PIs were unambiguously localized in the scaffolds by F-19 MRI throughout the whole experiment. Fluorescence was detected in the first 4days after transplantation only. Importantly, in vivo bioluminescence correlated with the F-19 MRI signal.ConclusionsWe developed a trimodal imaging platform for in vivo examination of transplanted PIs. Fluorescence imaging revealed instability of the fluorescent dye and its limited applicability for longitudinal in vivo studies. A correlation between the bioluminescence signal and the F-19 MRI signal indicated the fast clearance of PLGA-NPs from the transplantation site after cell death, which addresses a major issue with intracellular imaging labels. Therefore, the proposed PLGA-NP platform is reliable for reflecting the status of transplanted PIs in vivo.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30224 - Radiology, nuclear medicine and medical imaging
Result continuities
Project
<a href="/en/project/GA16-03156S" target="_blank" >GA16-03156S: Self-assembled polymeric nanostructures as bimodal magnetic resonance ultrasound contrast agents for imaging</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular imaging and biology
ISSN
1536-1632
e-ISSN
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Volume of the periodical
21
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
454-464
UT code for WoS article
000468363800008
EID of the result in the Scopus database
2-s2.0-85053230805