A novel model for in vivo quantification of immediate liver perfusion impairment after pancreatic islet transplantation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F19%3A00078731" target="_blank" >RIV/00023001:_____/19:00078731 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/19:10410372
Result on the web
<a href="https://www.tandfonline.com/doi/full/10.1080/19382014.2019.1651164" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/19382014.2019.1651164</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/19382014.2019.1651164" target="_blank" >10.1080/19382014.2019.1651164</a>
Alternative languages
Result language
angličtina
Original language name
A novel model for in vivo quantification of immediate liver perfusion impairment after pancreatic islet transplantation
Original language description
Instant Blood-Mediated Inflammatory Reaction (IBMIR) is a major cause of graft loss during pancreatic islet transplantation, leading to a low efficiency of this treatment method and significantly limiting its broader clinical use. Within the procedure, transplanted islets obstruct intrahepatic portal vein branches and consequently restrict blood supply of downstream lying liver tissue, resulting typically in ischemic necrosis. The extent of ischemic lesions is influenced by mechanical obstruction and inflammation, as well as subsequent recanalization and regeneration capacity of recipient liver tissue. Monitoring of immediate liver perfusion impairment, which is directly related to the intensity of post-transplant inflammation and thrombosis (IBMIR), is essential for improving therapeutic and preventive strategies to improve overall islet graft survival. In this study, we present a new experimental model enabling direct quantification of liver perfusion impairment after pancreatic islet transplantation using ligation of hepatic arteries followed by contrast-enhanced magnetic resonance imaging (MRI). The ligation of hepatic arteries prevents the contrast agent from circumventing the portal vein obstruction and enables to discriminate between well-perfused and non-perfused liver tissue. Here we demonstrate that the extent of liver ischemia reliably reflects the number of transplanted islets. This model represents a useful tool for in vivo monitoring of biological effect of IBMIR-alleviating interventions as well as other experiments related to liver ischemia. This technical paper introduces a novel technique and its first application in experimental animals. © 2019, © 2019 IKEM. Published with license by Taylor & Francis Group, LLC.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30213 - Transplantation
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Islets
ISSN
1938-2014
e-ISSN
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Volume of the periodical
11
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
129-140
UT code for WoS article
000485635900001
EID of the result in the Scopus database
2-s2.0-85072060960