Monitoring of amoxicilline and ceftazidime in the microdialysate of diabetic foot and serum by capillary electrophoresis with contactless conductivity detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F22%3A00082831" target="_blank" >RIV/00023001:_____/22:00082831 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/22:43922833
Result on the web
<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.202100366" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.202100366</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.202100366" target="_blank" >10.1002/elps.202100366</a>
Alternative languages
Result language
angličtina
Original language name
Monitoring of amoxicilline and ceftazidime in the microdialysate of diabetic foot and serum by capillary electrophoresis with contactless conductivity detection
Original language description
Determination of the broad-spectrum antibiotics amoxicilline (AMX) and ceftazidime (CTZ) in blood serum and microdialysates of the subcutaneous tissue of the lower limbs is performed using CE with contactless conductivity detection ((CD)-D-4). Baseline separation of AMX is achieved in 0.5 M acetic acid as the background electrolyte and separation of CTZ in 3.2 M acetic acid with addition of 13% v/v methanol. The CE-(CD)-D-4 determination is performed in a 25 mu m capillary with suppression of the EOF using INST-coating on an effective length of 18 cm and the attained migration time is 4.2 min for AMX and 4.4 min for CTZ. The analysis was performed using 20 mu l of serum and 15 mu l of microdialysate, treated by the addition of acetonitrile in a ratio of 1/3 v/v and the sample is injected into the capillary using the large volume sample stacking technique. The LOQ attained in the microdialysate is 148 ng/ml for AMX and 339 ng/ml for CTZ, and in serum 143 ng/ml for AMX and 318 ng/ml for CTZ. The CE-(CD)-D-4 method is employed for monitoring the passage of AMX and CTZ from the blood circulatory system into the subcutaneous tissue at the sites of diabetic ulceration in patients suffering from diabetic foot syndrome and also for measuring the pharmacokinetics following intravenous application of bolus antibiotic doses.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/NU20-01-00078" target="_blank" >NU20-01-00078: Rationalisation of ATB Therapy in Diabetic Foot Infection and its Impact on the Intestinal Microbiota</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
1522-2683
Volume of the periodical
43
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
1129-1139
UT code for WoS article
000783237500001
EID of the result in the Scopus database
2-s2.0-85128308575