Elevated plasma levels of cell-free mtDNA are associated with acute rejection following heart transplantation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F25%3A00086155" target="_blank" >RIV/00023001:_____/25:00086155 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/25:10504112

Result on the web

<a href="https://jab.zsf.jcu.cz/pdfs/jab/2025/03/01.pdf" target="_blank" >https://jab.zsf.jcu.cz/pdfs/jab/2025/03/01.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.32725/jab.2025.014" target="_blank" >10.32725/jab.2025.014</a>

Result language

angličtina

Original language name

Elevated plasma levels of cell-free mtDNA are associated with acute rejection following heart transplantation

Original language description

Acute rejection (AR) following heart transplantation (HTx) is a common complication, especially in the early post-HTx period. Mitochondrial DNA (mtDNA), released into circulation from stressed mitochondria, mimics ongoing immune activation and facilitates the release of pro-inflammatory substances. Our study aimed to assess cell-free mtDNA levels to identify early indicators of acute rejection progression. The absolute concentration of cf-mtDNA (cp/mu l) was measured in 77 adult patients using quantitative polymerase chain reaction. Blood samples (n = 300) were collected before their corresponding biopsy according to the timeline within the first year post-HTx. The median cf-mtDNA levels in samples with confirmed AR (n = 57) was higher compared to samples without diagnosed rejection (n = 210; Padj &lt; 0.01). When acute cellular (ACR; n = 39) and antibody-mediated rejection (AMR; n = 18) were analyzed separately, only AMR demonstrated higher levels compared to samples without diagnosed rejection (Padj = 0.02). The highest cf-mtDNA levels were detected in samples collected during early post-HTx complications compared to samples without rejection and AR samples (for both Padj &lt; 0.0001). Both ACR and AMR were observed throughout the one-year period, with the majority (3rd quartile) occurring during the first 200 days post-HTx. Post-HTx complications, such as graft dysfunction or acute kidney injury, were observed within the first 11 days, with the majority (71.4%) occurring within 5 days post-HTx. The presence of AR, and specifically AMR, is associated with elevated levels of cf-mtDNA. The increase in plasma cf-mtDNA levels strongly reflects the occurrence of early complications following HTx.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30104 - Pharmacology and pharmacy

Project

<a href="/en/project/NU22-01-00151" target="_blank" >NU22-01-00151: Prediction of cardiovascular risk and prognosis in patients with hypercholesterolaemia through genomic and metabolomic analysis</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of applied biomedicine

ISSN

1214-021X

e-ISSN

1214-0287

Volume of the periodical

23

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

10

Pages from-to

97-106

UT code for WoS article

001586816800001

EID of the result in the Scopus database

2-s2.0-105017724528