Cord Blood Extracellular Vesicles Analyzed by Flow Cytometry with Thresholding Using 405 nm or 488 nm Laser Leads to Concurrent Results
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023698%3A_____%2F21%3AN0000003" target="_blank" >RIV/00023698:_____/21:N0000003 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/21:10430980 RIV/00216208:11310/21:10430980
Result on the web
<a href="https://doi.org/10.3390/diagnostics11081320" target="_blank" >https://doi.org/10.3390/diagnostics11081320</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/diagnostics11081320" target="_blank" >10.3390/diagnostics11081320</a>
Alternative languages
Result language
angličtina
Original language name
Cord Blood Extracellular Vesicles Analyzed by Flow Cytometry with Thresholding Using 405 nm or 488 nm Laser Leads to Concurrent Results
Original language description
Extracellular vesicles (EVs) from liquid biopsies are extensively analyzed by flow cytometry, a technology that is continuously evolving. Thresholding utilizing a violet 405 nm laser side scatter (VSSC) has recently been implemented. Here, we collected set of large EV (1EV) samples from cord blood, which we analyzed using a standard flow cytometer improved via a 405 nm laser side scatter. Samples were analyzed using two distinct thresholding methods-one based on VSSC, and one based on VSSC combined with fluorescence thresholding on stained phosphatidylserine. Through these thresholding methods, we compared lEVs from pre-term births and control cord blood. Double-labeled 1EVs with platelet CD36+/CD41+, activated platelet CD41+ /CD62P+ and endothelial CD31+/CD105+ antibodies were used. Apart from comparing the two groups together, we also correlated measured 1EVs with the thresholding methods. We also correlated the results of this study with data analyzed in our previous study in which we used a conventional 488 nm laser SSC. We did not find any difference between the two cord blood groups. However, we found highly concurrent data via our correlation of the thresholding methods, with correlation coefficients ranging from 0.80 to 0.96 even though the numbers of detected lEVs differed between thresholding methods. In conclusion, our approaches to thresholding provided concurrent data and it seems that improving the cytometer with the use of a VSSC increases its sensitivity, despite not being particularly critical to the validity of flow cytometric studies that compare pathological and physiological conditions in liquid biopsies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30218 - General and internal medicine
Result continuities
Project
<a href="/en/project/NV17-31403A" target="_blank" >NV17-31403A: Diagostic potential of cellular microparticules in fetal inflammatory response syndrome.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
DIAGNOSTICS
ISSN
2075-4418
e-ISSN
2075-4418
Volume of the periodical
11
Issue of the periodical within the volume
8
Country of publishing house
CH - SWITZERLAND
Number of pages
8
Pages from-to
1320
UT code for WoS article
000688925700001
EID of the result in the Scopus database
2-s2.0-85112017092