Validation of RNA extraction procedures focused on micro RNA expression analysis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023728%3A_____%2F14%3A%230003054" target="_blank" >RIV/00023728:_____/14:#0003054 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Validation of RNA extraction procedures focused on micro RNA expression analysis

Original language description

Preparation of RNA is a crucial starting point in all expression analysis studies. The quality and specific template quantity of RNA can affect the amplification efficiency and also the final expression values. Authors have compared five different RNA extraction protocols from peripheral blood/PBMCs with the aim to define the most suitable method for the miRNA expression profiling experiments. They conclude that the yield and quality of total RNA as well as the portion of miRNAs prepared by the TRIzol-chloroform extraction procedure is sufficient for downstream expression profiling analysis.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FE - Other fields of internal medicine

OECD FORD branch

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Project

<a href="/en/project/NT12452" target="_blank" >NT12452: The significance of micro (mi)RNA in etiopathogenesis of myositis: application of miRNA expression profiling for monitoring of disease activity</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia biologica

ISSN

0015-5500

e-ISSN

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Volume of the periodical

59

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

4

Pages from-to

47-50

UT code for WoS article

000326215200006

EID of the result in the Scopus database

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