Expanding the citrullinome of synovial fibrinogen from rheumatoid arthritis patients

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023728%3A_____%2F19%3AN0000021" target="_blank" >RIV/00023728:_____/19:N0000021 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/19:10400666
Result on the web
<a href="https://doi.org/10.1016/j.jprot.2019.103484" target="_blank" >https://doi.org/10.1016/j.jprot.2019.103484</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jprot.2019.103484" target="_blank" >10.1016/j.jprot.2019.103484</a>

Result language
angličtina
Original language name
Expanding the citrullinome of synovial fibrinogen from rheumatoid arthritis patients
Original language description
Citrullination is a post-translational protein modification, which is associated with inflammation in general and is thought to play an important pathogenic role in rheumatoid arthritis (RA). Here a mass spectrometry-based proteomics approach was applied to identify citrullination sites in synovial fluid fibrinogen from four RA patients. In general, high disease activity correlated with increased number of identified citrullination sites and higher relative citrulline occupancy. Altogether, 23 sites were identified, of which 9 have not been previously reported to be citrullinated in vivo. Citrullination at site alpha 84, alpha 123, alpha 129, alpha 547, alpha 573, alpha 591, beta 334 and gamma 134 was identified in more than one patient, and these positions were therefore regarded as hotspots. Following citrullination of fibrinogen in vitro using human recombinant peptidylarginine deiminase 2 (PAD2), a total of 46 citrullination sites were identified, including 6 hitherto unreported in vitro citrullination sites. Twenty-two out of the 23 citrullination sites identified in vivo were also detected in vitro, supporting the validity of the identifications
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30226 - Rheumatology

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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