Aggregated neutrophil extracellular traps occlude Meibomian glands during ocular surface inflammation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023728%3A_____%2F21%3AN0000007" target="_blank" >RIV/00023728:_____/21:N0000007 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/21:10428929

Result on the web

<a href="https://doi.org/10.1016/j.jtos.2020.12.005" target="_blank" >https://doi.org/10.1016/j.jtos.2020.12.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jtos.2020.12.005" target="_blank" >10.1016/j.jtos.2020.12.005</a>

Result language

angličtina

Original language name

Aggregated neutrophil extracellular traps occlude Meibomian glands during ocular surface inflammation

Original language description

Meibomian gland dysfunction (MGD) is one of the leading causes of evaporative dry eye disease. Meibomian glands at the eyelid secrete lipids that prevent evaporation of the aqueous tear film. The pathogenesis of obstructive MGD is incompletely understood to date. Herein, we aim to investigate the pathogenesis of obstructive MGD using murine and human samples with various forms of ocular surface inflammation. The presence of Neutrophil extracellular Traps (NETs) was detected with immunofluorescence analysis of ocular surface discharge and biopsy samples from patients with blepharitis. Tear fluid from patients with MGD and blepharitis were evaluated for the presence of inflammatory mediators using bead based immunoassay. Murine model of allergic eye disease (AED) was performed to investigate the role of NETs in MG occlusion. We show that the ocular discharge from patients with blepharitis contains aggregated neutrophil extracellular traps (aggNETs). Furthermore, the ducts of human Meibomian glands affected by blepharitis were largely congested by aggNETs. Tear fluid from patients with MGD showed elevated neutrophil chemoattractants (C5a, IL6, IL8 and IL18). C5a and IL8 correlated with the degree of deficiency of tear fluid. In the murine model of allergic eye disease (AED), aggNETs accumulated in the MG leading to occlusion of their ducts and the retrograde pent-up of the fluid followed by acinar atrophy. Constraining aggNET formation by genetic or pharmacological inhibition of peptidyl arginine deiminase type 4 (PADI4) effectively reduced MG damage. Conclusion: We conclude that aggNETs 

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30226 - Rheumatology

Project

—

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Ocular Surface

ISSN

1542-0124

e-ISSN

1937-5913

Volume of the periodical

2021

Issue of the periodical within the volume

20

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

1-12

UT code for WoS article

000647679000001

EID of the result in the Scopus database

2-s2.0-85098763809