DNA methylation validation methods: a coherent review with practical comparison
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F19%3A00012760" target="_blank" >RIV/00023736:_____/19:00012760 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/19:10400796
Result on the web
<a href="https://doi.org/10.1186/s12575-019-0107-z" target="_blank" >https://doi.org/10.1186/s12575-019-0107-z</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12575-019-0107-z" target="_blank" >10.1186/s12575-019-0107-z</a>
Alternative languages
Result language
angličtina
Original language name
DNA methylation validation methods: a coherent review with practical comparison
Original language description
Here, we present a practical overview of four commonly used validation methods for DNA methylation assessment: methylation specific restriction endonucleases (MSRE) analysis, pyrosequencing, methylation specific high-resolution DNA melting (MS-HRM) and quantitative methylation specific polymerase chain reaction (qMSP). Using these methods, we measured DNA methylation levels of three loci in human genome among which one was highly methylated, one intermediately methylated and one unmethylated. We compared the methods in terms of primer design demands, methods’ feasibility, accuracy, time and money consumption, and usability for clinical diagnostics.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30205 - Hematology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biological procedures online
ISSN
1480-9222
e-ISSN
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Volume of the periodical
21
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
"art. no. 19"
UT code for WoS article
000488472100001
EID of the result in the Scopus database
2-s2.0-85073072577