All -Trans Retinoic Acid Fosters the Multifarious U87MG Cell Line as a Model of Glioblastoma
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023752%3A_____%2F21%3A43920610" target="_blank" >RIV/00023752:_____/21:43920610 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/21:43921739
Result on the web
<a href="https://www.mdpi.com/2076-3425/11/6/812" target="_blank" >https://www.mdpi.com/2076-3425/11/6/812</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/brainsci11060812" target="_blank" >10.3390/brainsci11060812</a>
Alternative languages
Result language
angličtina
Original language name
All -Trans Retinoic Acid Fosters the Multifarious U87MG Cell Line as a Model of Glioblastoma
Original language description
Glioblastoma multiforme (GBM) is a primary brain cancer of poor prognosis, with existing treatments remaining essentially palliative. Current GBM therapy fails due to rapid reappearance of the heterogeneous neoplasm, with models suggesting that the recurrent growth is from treatment- resistant glioblastoma stem-like cells (GSCs). Whether GSCs depend on survival/proliferative cues from their surrounding microenvironmental niche, particularly surrounding the leading edge after treatment remains unknown. Simulating human GBM in the laboratory relies on representative cell lines and xenograft models for translational medicine. Due to U87MG source discrepancy and differential proliferation responses to retinoic acid treatment, this study highlights the challenges faced by laboratory scientists working with this representative GBM cell line. Investigating the response to all trans-retinoic acid (ATRA) revealed its sequestering of the prominin-1 stem cell marker. ICAM-1 universally present throughout U87MG was enhanced by ATRA, of interest for chemotherapy targeting studies. ATRA triggered diverse expression patterns of long non-coding RNAs PARTICLE and GAS5 in the leading edge and established monolayer growth zone microenvironment. Karyotyping confirmed the female origin of U87MG sourced from Europe. Passaging U87MG revealed the presence of chromosomal anomalies reflective of structural genomic alterations in this glioblastoma cell line. All evidence considered, this study exposes further phenotypic nuances of U87MG which may belie researchers seeking data contributing towards the elusive cure or GBM.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30502 - Other medical science
Result continuities
Project
—
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Brain Sciences
ISSN
2076-3425
e-ISSN
—
Volume of the periodical
11
Issue of the periodical within the volume
6
Country of publishing house
CH - SWITZERLAND
Number of pages
14
Pages from-to
"Article Number: 812"
UT code for WoS article
000667840700001
EID of the result in the Scopus database
2-s2.0-85108878277