Quantification of chitooligosaccharides by FACE method: Determination of combinatory effects of mouse chitinases
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023884%3A_____%2F20%3A00008776" target="_blank" >RIV/00023884:_____/20:00008776 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S221501612030100X" target="_blank" >https://www.sciencedirect.com/science/article/pii/S221501612030100X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.mex.2020.100881" target="_blank" >10.1016/j.mex.2020.100881</a>
Alternative languages
Result language
angličtina
Original language name
Quantification of chitooligosaccharides by FACE method: Determination of combinatory effects of mouse chitinases
Original language description
Fluorophore-assisted carbohydrate electrophoresis (FACE) enables detection and quantification of degradation products from artificial and natural chitin substrates such as 4-NP-(GlcNAc)2, (GlcNAc)4 and colloidal chitin. The FACE method has been improved by our group for analysis of chitooligosaccharides in the presence of several buffer systems commonly used in the biochemical evaluation of chitinolytic activities of enzymes at pH 2.0–8.0. FACE is a very sensitive technique detecting picomolar amounts of molecules. We optimized the detection conditions as follows: exposure type, precision; sensitivity, high resolution; exposure time, 5 s. We evaluated the (GlcNAc)2 levels using a standard curve that allows chitooligosaccharides quantification at up to 10 nmol amounts. Using the method presented here, the chitinolytic properties of different chitinases can be compared directly. Serratia chitinase A (ChiA) and chitinase B (ChiB), two well-studied bacterial chitinases, have been shown by HPLC to have a synergistic effect on the chitin degradation rate. Using the FACE method, we determined the combinatory effects of mouse chitotriosidase (Chit1) and acidic mammalian chitinase (AMCase) in natural chitin substrates processing. • FACE is a simple and quantitative method. • Our improved procedure enables the quantification of chitooligosaccharides produced by chitinases at pH 2.0–8.0. • FACE is able to quantify chitooligosaccharides at up to 10 nmol amounts.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30101 - Human genetics
Result continuities
Project
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Continuities
N - Vyzkumna aktivita podporovana z neverejnych zdroju
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
MethodsX
ISSN
2215-0161
e-ISSN
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Volume of the periodical
7
Issue of the periodical within the volume
April
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
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UT code for WoS article
000608187300003
EID of the result in the Scopus database
2-s2.0-85083507758