Robust chitinolytic activity of crab-eating monkey (Macaca fascicularis) acidic chitinase under a broad pH and temperature range

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023884%3A_____%2F21%3A00009106" target="_blank" >RIV/00023884:_____/21:00009106 - isvavai.cz</a>

Result on the web

<a href="https://www-nature-com.ezproxy.lib.cas.cz/articles/s41598-021-95010-w" target="_blank" >https://www-nature-com.ezproxy.lib.cas.cz/articles/s41598-021-95010-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-021-95010-w" target="_blank" >10.1038/s41598-021-95010-w</a>

Result language

angličtina

Original language name

Robust chitinolytic activity of crab-eating monkey (Macaca fascicularis) acidic chitinase under a broad pH and temperature range

Original language description

Diet of the crab-eating monkey (Macaca fascicularis) consists of both plants and animals, including chitin-containing organisms such as crabs and insects. This omnivorous monkey has a high expression of acidic chitinase (CHIA) in the stomach and here, we report on its enzymatic properties under different conditions. When we compared with Mus musculus CHIA (Mm-CHIA), Macaca fascicularis CHIA (Mf-CHIA) exhibits higher chitinolytic activity at broad pH (1.0-7.0) and temperature (30-70 ?) range. Interestingly, at its optimum pH (5.0), Mf-CHIA showed the highest activity at 65 degrees C while maintaining it at robust levels between 50 and 70 degrees C. The degradation efficiency of Mf-CHIA was superior to Mm-CHIA toward both polymeric chitin as well as an artificial chromogenic substrate. Our results show that unique features of Mf-CHIA including its thermostability warrant the nomination of this enzyme for potential agricultural and biomedical applications.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30102 - Immunology

Project

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Continuities

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

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UT code for WoS article

000681066000061

EID of the result in the Scopus database

2-s2.0-85111547720