Sequence analysis of the 5'-terminal part of GLU-D1 locus of landraces and old cultivars of wheat (Triticum aestivum)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F00%3A107" target="_blank" >RIV/00027006:_____/00:107 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Sequence analysis of the 5'-terminal part of GLU-D1 locus of landraces and old cultivars of wheat (Triticum aestivum)

Original language description

We have analyzed 380bp long N-terminal parts of Glu-D1x alleles from several lines of landraces and old cultivars of wheat (Triticum aestivum L.) with the aim to verify, whether alleles characterized as identical according to their mobility in SDS-PAGE are encoded always by the same DNA sequences. European landraces and old cultivars were chosen for the study as a potential source of higher genetic variability as compared with modern wheat cultivars. We have not found any changes in comparison with known data in the sequences of Dx3 and Dx4 alleles, which in fact were rare in the available set of landraces and old cultivars. Three point mutations were found together in Dx2 and Dx5 alleles of landraces and old cultivars, no one in the standard wheat cultivars Chinese Spring and Bezostaya1. Sequencing analysis can be another suitable technique for study of glutenin alleles complementing SDS-PAGE.

Czech name

Sekvenční analýza 5'- části GLU-D1 pšenice

Czech description

We have analyzed 380bp long N-terminal parts of Glu-D1x alleles from several lines of landraces and old cultivars of wheat (Triticum aestivum L.) with the aim to verify, whether alleles characterized as identical according to their mobility in SDS-PAGE are encoded always by the same DNA sequences. European landraces and old cultivars were chosen for the study as a potential source of higher genetic variability as compared with modern wheat cultivars. We have not found any changes in comparison with known data in the sequences of Dx3 and Dx4 alleles, which in fact were rare in the available set of landraces and old cultivars. Three point mutations were found together in Dx2 and Dx5 alleles of landraces and old cultivars, no one in the standard wheat cultivars Chinese Spring and Bezostaya1. Sequencing analysis can be another suitable technique for study of glutenin alleles complementing SDS-PAGE.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GE - Plant cultivation

OECD FORD branch

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Project

<a href="/en/project/GA521%2F97%2F0222" target="_blank" >GA521/97/0222: Molecular analysis of gliadin and glutenin genes in wheat and relative species</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2000

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cereal Research Communications

ISSN

0133-3720

e-ISSN

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Volume of the periodical

28

Issue of the periodical within the volume

4

Country of publishing house

HU - HUNGARY

Number of pages

3

Pages from-to

414-416

UT code for WoS article

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EID of the result in the Scopus database

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