Characterisation of variability at Glu-3 loci in some European wheat obsolete cultivars and landraces using PCR

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F01%3A105" target="_blank" >RIV/00027006:_____/01:105 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Characterisation of variability at Glu-3 loci in some European wheat obsolete cultivars and landraces using PCR

Original language description

A set of European obsolete wheat cultivars and landraces was evaluated using molecular markers. Three primer pairs were used to amplify LMW-GS coding sequences. To enrich the current gene-pool, modem Czech wheat cultivars were used for the assessment ofthe potential of old genotypes. Thirty-eight amplification products of different sizes were recorded across the evaluated landraces and cultivars. Specific PCR profiles were amplified for each obsolete cultivar and landrace and for all evaluated modern Czech cultivars. Analysis revealed differences between modern and obsolete cultivars and landraces in the representation of LMW-GS al1eles. High variability was found within evaluated obsolete cultivars and landraces. Sufficient evidence has been gained to suggest that obsolete cultivars and landraces can represent a source of alternative LMW-GS al1eles for the wheat breeding programs.

Czech name

Charakterizace varaiability Glu-3 u některých evropských kultivarů zakrslé pšenice pomocí PCR

Czech description

A set of European obsolete wheat cultivars and landraces was evaluated using molecular markers. Three primer pairs were used to amplify LMW-GS coding sequences. To enrich the current gene-pool, modem Czech wheat cultivars were used for the assessment ofthe potential of old genotypes. Thirty-eight amplification products of different sizes were recorded across the evaluated landraces and cultivars. Specific PCR profiles were amplified for each obsolete cultivar and landrace and for all evaluated modern Czech cultivars. Analysis revealed differences between modern and obsolete cultivars and landraces in the representation of LMW-GS al1eles. High variability was found within evaluated obsolete cultivars and landraces. Sufficient evidence has been gained to suggest that obsolete cultivars and landraces can represent a source of alternative LMW-GS al1eles for the wheat breeding programs.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA521%2F97%2F0222" target="_blank" >GA521/97/0222: Molecular analysis of gliadin and glutenin genes in wheat and relative species</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2001

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cereal Research Communications

ISSN

0133-3720

e-ISSN

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Volume of the periodical

29

Issue of the periodical within the volume

1

Country of publishing house

HU - HUNGARY

Number of pages

9

Pages from-to

205-213

UT code for WoS article

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EID of the result in the Scopus database

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