Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F03%3A33" target="_blank" >RIV/00027006:_____/03:33 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population

Original language description

The polymerase chain reaction (PCR) assay was successfully used to identify Cydia pomonella granulovirus (CpGV) in larvae of Cydia pomonella L. (codling moth). PCR with the primers CpGV-2A1/CpGV-2B and CpGV-3A/CpGV-3B was found suitable for detection ofCpGV. The primers Cp-I/Cp-II and Cp-III/Cp-IV were able to identify the transposable element TCp3.2 in C. pomonella larvae. The presence of CpGV in the larvae from orchards, which had been infected with CpGV was tested during 2 years post infection. (pi.). CpGV was found in as many as 15% of the surviving larvae 1 year p.i. in one location. The virus was not detected in CpGV-infected orchards 2 years p.i. or in natural C. pomonella populations. This result suggests a poor persistence of CpGV in surviving C. pomonella individuals and its slow spread in a natural host population. One the other band, the presence of a transposable element, transposon TCp3.2 may correlate with virus redistribution in this insect population.

Czech name

Metody PCR pro detekci Cydia pomonella granulovirus v populaci Cydia pomonella

Czech description

PCR pro detekci granuloviru Cydia pomonella (CpGV) v larvách Cydia pomonella L. (obaleč jablečný) byla vyvinuta. Dále byly vyvinuty primery pro detekci transposonu TCp3.2 v larvách C. pomonella.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Virologica

ISSN

0001-723X

e-ISSN

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Volume of the periodical

47

Issue of the periodical within the volume

3

Country of publishing house

SK - SLOVAKIA

Number of pages

5

Pages from-to

153-157

UT code for WoS article

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EID of the result in the Scopus database

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