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Sample processing effect on polymerase chain reaction (PCR)used for identification of Campylobacter jejuni.

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A01%3A_3575" target="_blank" >RIV/00027006:_____/04:01:_3575 - isvavai.cz</a>

  • Alternative codes found

    RIV/60461373:_____/04:01:_3575

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Sample processing effect on polymerase chain reaction (PCR)used for identification of Campylobacter jejuni.

  • Original language description

    Model patterns gout Campylobacter jejuni for PCR were prepared quick and simple procedure, who contained skimmed cellule, preparation of proteinasy K, prídavek Chelex 100 and lysy created boiling point.

  • Czech name

    Stanovení vlivu faktoru zpracování vzorku na identifikaci C. jejuni.

  • Czech description

    Modelové vzorky DNA Campylobacter jejuni pro PCR byly pripraveny rychlým a jednoduchým postupem, který zahrnoval odstredení bunek, prídavek proteinasy K, prídavek Chelex 100 a lysy zpusobenou varem. PCR byla založena na amplifikaci ruzných sekvencí 16SrRNA genu za použití Tth DNA polymerasy. PCR produkty byly vizualizovány na agarosovém gelu po elektroforéze. Stanovení umožnilo detekci 10 CFU/mL Campylobacter jejuni ve fysiologickém roztoku a 1000 CFU/mL v mediu podle Parka a Sanderse.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/1B44068" target="_blank" >1B44068: Development and validation of methods for increasing food quality and food safety using molecular biology technologies in agreement with EU regulations.</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Folia Microbiologica 2004

  • ISSN

    0015-5632

  • e-ISSN

  • Volume of the periodical

    49

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    4

  • Pages from-to

    693-697

  • UT code for WoS article

  • EID of the result in the Scopus database